• Immunohistochemistry analysis of paraffin-embedded mouse kidney using CARD10 antibody (STJ29506) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver damage using CARD10 antibody (STJ29506) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Rat kidney, using CARD10 antibody (STJ29506) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of Mouse kidney, using CARD10 antibody (STJ29506) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CARD10 antibody (863-1032) (STJ29506)

SKU:
STJ29506

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CARD10 (863-1032) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CARD10
Gene ID: 29775
Uniprot ID: CAR10_HUMAN
Immunogen Region: 863-1032
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 863-1032 of human CARD10 (NP_055365.2).
Immunogen Sequence: LLDLPSSRLDFQVCPAESLS GEELCPSSAPGAPKAQPATP GLGSRIRAIQESVGKKHCLL ELGARGVRELVQNEIYPIVI HVEVTEKNVREVRGLLGRPG WRDSELLRQCRGSEQVLWGL PCSWVQVPAHEWGHAEELAK VVRGRILQEQARLVWVECGS SRGCPSSSEA
Tissue Specificity Detected in adult heart, kidney and liver.lower levels in intestine, placenta, muscle and lung. Also found in fetal lung, liver and kidney.
Function Scaffold protein that plays an important role in mediating the activation of NF-kappa-B via BCL10 or EGFR.
Protein Name Caspase Recruitment Domain-Containing Protein 10
Card-Containing Maguk Protein 3
Carma 3
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Caspase Recruitment Domain-Containing Protein 10 antibody
Anti-Card-Containing Maguk Protein 3 antibody
Anti-Carma 3 antibody
Anti-CARD10 antibody
Anti-CARMA3 antibody

Information sourced from Uniprot.org

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