Western blot analysis of extracts of various cell lines, using Calpain small subunit 1 rabbit monoclonal antibody (STJ11102385) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-CAPNS1 antibody (1-100) [S5MR] (STJ11102385)

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STJ11102385
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Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAPNS1
Gene ID: 826
Uniprot ID: CPNS1_HUMAN
Immunogen Region: 1-100
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human Calpain small subunit 1 (P04632).
Immunogen Sequence: MFLVNSFLKGGGGGGGGGGG LGGGLGNVLGGLISGAGGGG GGGGGGGGGGGGGGGGTAMR ILGGVISAISEAAAQYNPEP PPPRTHYSNIEANESEEVRQ
Function Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Essential for embryonic development.
Protein Name Calpain Small Subunit 1
Css1
Calcium-Activated Neutral Proteinase Small Subunit
Canp Small Subunit
Calcium-Dependent Protease Small Subunit
Cdps
Calcium-Dependent Protease Small Subunit 1
Calpain Regulatory Subunit
Database Links Reactome: R-HSA-1474228
Reactome: R-HSA-6809371
Reactome: R-HSA-8862803
Cellular Localisation Cytoplasm
Cell Membrane
Translocates To The Plasma Membrane Upon Calcium Binding
Alternative Antibody Names Anti-Calpain Small Subunit 1 antibody
Anti-Css1 antibody
Anti-Calcium-Activated Neutral Proteinase Small Subunit antibody
Anti-Canp Small Subunit antibody
Anti-Calcium-Dependent Protease Small Subunit antibody
Anti-Cdps antibody
Anti-Calcium-Dependent Protease Small Subunit 1 antibody
Anti-Calpain Regulatory Subunit antibody
Anti-CAPNS1 antibody
Anti-CAPN4 antibody
Anti-CAPNS antibody

Information sourced from Uniprot.org

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