• Immunofluorescence analysis of NIH-3T3 cells using CAPN5 antibody (STJ29564) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CAPN5 antibody (STJ29564) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-CAPN5 antibody (361-640) (STJ29564)

SKU:
STJ29564

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CAPN5 (361-640) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAPN5
Gene ID: 726
Uniprot ID: CAN5_HUMAN
Immunogen Region: 361-640
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 361-640 of human CAPN5 (NP_004046.2).
Immunogen Sequence: GAWTLHEDPRQNRGGGCINH KDTFFQNPQYIFEVKKPEDE VLICIQQRPKRSTRREGKGE NLAIGFDIYKVEENRQYRMH SLQHKAASSIYINSRSVFLR TDQPEGRYVIIPTTFEPGHT GEFLLRVFTDVPSNCRELRL DEPPHTCWSSLCGYPQLVTQ VHVLGAAGLKDSPTGANSYV IIKCEGDKVRSAVQKGTSTP EYNVKGIFYRKKLSQPITVQ VWNHRVLKDEFLGQVHLKA
Tissue Specificity Expressed in many tissues. Strong expression in the photoreceptor cells of the retina, with a punctate pattern of labeling over the nuclei and inner segments with less expression along the other segments and outer plexiform layer.
Function Calcium-regulated non-lysosomal thiol-protease.
Protein Name Calpain-5
Calpain Htra-3
New Calpain 3
Ncl-3
Database Links Reactome: R-HSA-1474228
Alternative Antibody Names Anti-Calpain-5 antibody
Anti-Calpain Htra-3 antibody
Anti-New Calpain 3 antibody
Anti-Ncl-3 antibody
Anti-CAPN5 antibody
Anti-NCL3 antibody

Information sourced from Uniprot.org

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