Western blot analysis of extracts of various cell lines, using Calpain 2 Rabbit monoclonal antibody (STJ11101989) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CAPN2 antibody (600-700) [S9MR] (STJ11101989)

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STJ11101989
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:6000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAPN2
Gene ID: 824
Uniprot ID: CAN2_HUMAN
Immunogen Region: 600-700
Specificity: A synthetic peptide corresponding to a sequence within amino acids 600-700 of human Calpain 2 (P17655).
Immunogen Sequence: LWTKIQKYQKIYREIDVDRS GTMNSYEMRKALEEAGFKMP CQLHQVIVARFADDQLIIDF DNFVRCLVRLETLFKIFKQL DPENTGTIELDLISWLCFSV L
Tissue Specificity Ubiquitous.
Function Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'. Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs.
Protein Name Calpain-2 Catalytic Subunit
Calcium-Activated Neutral Proteinase 2
Canp 2
Calpain M-Type
Calpain Large Polypeptide L2
Calpain-2 Large Subunit
Millimolar-Calpain
M-Calpain
Database Links Reactome: R-HSA-1474228
Reactome: R-HSA-8862803
Cellular Localisation Cytoplasm
Cell Membrane
Translocates To The Plasma Membrane Upon Ca(2+) Binding
Alternative Antibody Names Anti-Calpain-2 Catalytic Subunit antibody
Anti-Calcium-Activated Neutral Proteinase 2 antibody
Anti-Canp 2 antibody
Anti-Calpain M-Type antibody
Anti-Calpain Large Polypeptide L2 antibody
Anti-Calpain-2 Large Subunit antibody
Anti-Millimolar-Calpain antibody
Anti-M-Calpain antibody
Anti-CAPN2 antibody
Anti-CANPL2 antibody

Information sourced from Uniprot.org

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