| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Calpain-1 catalytic subunit is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 ELISA 1:5000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CAPN1 |
| Gene ID: | 823 |
| Uniprot ID: | CAN1_HUMAN |
| Specificity: | CAN1 Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: | Synthesized peptide derived from part of the human protein |
| Post Translational Modifications | Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms. |
| Function | Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409'. Cleaves and activates caspase-7 (CASP7). |
| Protein Name | Calpain-1 Catalytic Subunit Calcium-Activated Neutral Proteinase 1 Canp 1 Calpain Mu-Type Calpain-1 Large Subunit Cell Proliferation-Inducing Gene 30 Protein Micromolar-Calpain Mucanp |
| Database Links | Reactome: R-HSA-1474228 Reactome: R-HSA-6798695 Reactome: R-HSA-6809371 Reactome: R-HSA-8862803 |
| Cellular Localisation | Cytoplasm Cell Membrane Translocates To The Plasma Membrane Upon Ca(2+) Binding In Granular Keratinocytes And In Lower Corneocytes Colocalizes With Flg And Flg2 |
| Alternative Antibody Names | Anti-Calpain-1 Catalytic Subunit antibody Anti-Calcium-Activated Neutral Proteinase 1 antibody Anti-Canp 1 antibody Anti-Calpain Mu-Type antibody Anti-Calpain-1 Large Subunit antibody Anti-Cell Proliferation-Inducing Gene 30 Protein antibody Anti-Micromolar-Calpain antibody Anti-Mucanp antibody Anti-CAPN1 antibody Anti-CANPL1 antibody Anti-PIG30 antibody |
Information sourced from Uniprot.org