| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/IHC |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-CAPN1 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
WB-0.3-1µg/mlIHC-5µg/mlELISA-antibody detection limit dilution 1:32000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
CAPN1 |
| Gene ID: |
823 |
| Uniprot ID: |
CAN1_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_005177.2 |
| Specificity: |
Reported variants represent identical protein: NP_001185797.1, NP_005177.2, NP_001185798.1. |
| Immunogen Sequence: |
SEKSAGTVELDD |
| Post Translational Modifications | Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms. |
| Function | Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409'. Cleaves and activates caspase-7 (CASP7). |
| Protein Name | Calpain-1 Catalytic SubunitCalcium-Activated Neutral Proteinase 1Canp 1Calpain Mu-TypeCalpain-1 Large SubunitCell Proliferation-Inducing Gene 30 ProteinMicromolar-CalpainMucanp |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-6798695Reactome: R-HSA-6809371Reactome: R-HSA-8862803 |
| Cellular Localisation | CytoplasmCell MembraneTranslocates To The Plasma Membrane Upon Ca(2+) BindingIn Granular Keratinocytes And In Lower CorneocytesColocalizes With Flg And Flg2 |
| Alternative Antibody Names | Anti-Calpain-1 Catalytic Subunit antibodyAnti-Calcium-Activated Neutral Proteinase 1 antibodyAnti-Canp 1 antibodyAnti-Calpain Mu-Type antibodyAnti-Calpain-1 Large Subunit antibodyAnti-Cell Proliferation-Inducing Gene 30 Protein antibodyAnti-Micromolar-Calpain antibodyAnti-Mucanp antibodyAnti-CAPN1 antibodyAnti-CANPL1 antibodyAnti-PIG30 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and Calpain 1 knockout (KO) HeLa cells, using [KO Validated] Calpain 1 Rabbit polyclonal antibody (STJ119979) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98764/382816/STJ119979_1__25837.1713151311.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Calpain 1 knockout (KO) HeLa cells, using [KO Validated] Calpain 1 Rabbit polyclonal antibody (STJ119979) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")