• Immunofluorescence analysis of NIH/3T3 cells using Calpain 1 antibody (STJ22880) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using Calpain 1 antibody (STJ22880) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Calpain 1 Rabbit polyclonal antibody (STJ22880) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Calpain 1 antibody (STJ22880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CAPN1 antibody (355-530) (STJ22880)

SKU:
STJ22880

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CAPN1 (355-530) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAPN1
Gene ID: 823
Uniprot ID: CAN1_HUMAN
Immunogen Region: 355-530
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 355-530 of human Calpain 1 (NP_005177.2).
Immunogen Sequence: PDALKSRTIRKWNTTLYEGT WRRGSTAGGCRNYPATFWVN PQFKIRLDETDDPDDYGDRE SGCSFVLALMQKHRRRERRF GRDMETIGFAVYEVPPELVG QPAVHLKRDFFLANASRARS EQFINLREVSTRFRLPPGEY VVVPSTFEPNKEGDFVLRFF SEKSAGTVELDDQIQA
Tissue Specificity Ubiquitous.
Post Translational Modifications Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms.
Function Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409'. Cleaves and activates caspase-7 (CASP7).
Protein Name Calpain-1 Catalytic Subunit
Calcium-Activated Neutral Proteinase 1
Canp 1
Calpain Mu-Type
Calpain-1 Large Subunit
Cell Proliferation-Inducing Gene 30 Protein
Micromolar-Calpain
Mucanp
Database Links Reactome: R-HSA-1474228
Reactome: R-HSA-6798695
Reactome: R-HSA-6809371
Reactome: R-HSA-8862803
Cellular Localisation Cytoplasm
Cell Membrane
Translocates To The Plasma Membrane Upon Ca(2+) Binding
In Granular Keratinocytes And In Lower Corneocytes
Colocalizes With Flg And Flg2
Alternative Antibody Names Anti-Calpain-1 Catalytic Subunit antibody
Anti-Calcium-Activated Neutral Proteinase 1 antibody
Anti-Canp 1 antibody
Anti-Calpain Mu-Type antibody
Anti-Calpain-1 Large Subunit antibody
Anti-Cell Proliferation-Inducing Gene 30 Protein antibody
Anti-Micromolar-Calpain antibody
Anti-Mucanp antibody
Anti-CAPN1 antibody
Anti-CANPL1 antibody
Anti-PIG30 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance