• STJ73413 (0. 3µg/ml) staining of U937 (A) and Human Spleen (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Anti-CAPG (0. 3µg/ml) staining of Periheral Blood Lymphocytes (STJ73408 lane A) , STJ73413 lane B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ73413 (0. 1µg/ml) staining of Mouse (A) and Rat Spleen (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ73413 (0. 3µg/ml) staining of Pig Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CAPG antibody (205-217) (STJ73413)

SKU:
STJ73413-100

£312.50
Shipping:
Free Shipping
Current Stock:
Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Dog/Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-CAPG (205-217) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 38kDa band observed in lysates of cell line U937 and of Human, Mouse and Rat Spleen (calculated MW of 38.5kDa according to NP_001738.2). In Peripheral Blood Lymphocytes, the ob
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: CAPG
Gene ID: 822
Uniprot ID: CAPG_HUMAN
Immunogen Region: 205-217
Accession Number: NP_001738.2
Specificity: This antibody is expected to recognize isoform 1 (NP_001738.2) only. Reported variants represent identical protein: NP_001243068.1, NP_001738.2.
Immunogen Sequence: KAQVEIVTDGEEP
Post Translational Modifications The N-terminus is blocked.
Function Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.
Protein Name Macrophage-Capping Protein
Actin Regulatory Protein Cap-G
Cellular Localisation Nucleus
Cytoplasm
Melanosome
Cell Projection
Lamellipodium
Ruffle
In Macrophages
May Be Predominantly Cytoplasmic
Nuclear Localization Was Observed In Fibroblasts
Present At The Membrane-Cytoplasm Interface
In Activated Macrophages
Concentrated In The Ruffles Of The Leading Lamellipodia
Alternative Antibody Names Anti-Macrophage-Capping Protein antibody
Anti-Actin Regulatory Protein Cap-G antibody
Anti-CAPG antibody
Anti-AFCP antibody
Anti-MCP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance