| Host: | Goat |
| Applications: | WB/IHC-F/IHC-P/IF |
| Reactivity: | Human/Rat/Mouse/Monkey/Canine |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Calnexin (550aa C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: | This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: | 2 mg/mL |
| Dilution Range: | WB 1:500-1:5000IF 1:50-1:500IHC-P 1:200-1:1000IHC-F 1:200-1:1000Martins-Marques T Costa MC Catarino S et al. EMBO Rep 2022 May PMID: 35593040Sacramento JF Olea E Ribeiro MJ et al. J Physiol 2019 Aug. PMID: 31426127Ribot |
| Storage Instruction: | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Gene Symbol: | CANX |
| Gene ID: | 821 |
| Uniprot ID: | CALX_HUMAN |
| Immunogen Region: | 550aa C-Term |
| Accession Number: | ENSG00000127022 |
| Specificity: | Detects a band of 90 kDa by Western blot in the following human (293A, primary fibroblasts, HaCat, HeLa, HMEC-1, Jurkat, MNT1, U-118, rat (TR-iBRB) , mouse (3T3, AtT-20, Hepa, Raw264.7) , monkey (COS-7) and canine (D17) whole cell lysates. |
| Immunogen: | Purified recombinant peptide within residues 550 aa to the C-terminus of human CANX produced in E. coli. |
| Immunogen Sequence: | EFKSDAEEDGGTVSQEEEDR KPKAEEDEILNRSPRNRKPR RE |
| Post Translational Modifications | Phosphorylated at Ser-564 by MAPK3/ERK1. Phosphorylation by MAPK3/ERK1 increases its association with ribosomes. Palmitoylation by DHHC6 leads to the preferential localization to the perinuclear rough ER. It mediates the association of calnexin with the ribosome-translocon complex (RTC) which is required for efficient folding of glycosylated proteins. Ubiquitinated, leading to proteasomal degradation. Probably ubiquitinated by ZNRF4. |
| Function | Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse. |
| Protein Name | CalnexinIp90Major Histocompatibility Complex Class I Antigen-Binding Protein P88P90 |
| Database Links | Reactome: R-HSA-168316Reactome: R-HSA-2132295Reactome: R-HSA-8984722Reactome: R-HSA-901042Reactome: R-HSA-9020956Reactome: R-HSA-9683686Reactome: R-HSA-9694548Reactome: R-HSA-983170 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type I Membrane ProteinMitochondrion MembraneMelanosome MembraneIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvThe Palmitoylated Form Preferentially Localizes To The Perinuclear Rough ErLocalizes To Endoplasmic Reticulum Mitochondria-Associated Membrane (Mams) That Connect The Endoplasmic Reticulum And The Mitochondria |
| Alternative Antibody Names | Anti-Calnexin antibodyAnti-Ip90 antibodyAnti-Major Histocompatibility Complex Class I Antigen-Binding Protein P88 antibodyAnti-P90 antibodyAnti-CANX antibody |
Information sourced from Uniprot.org
