• Immunohistochemistry analysis of Calreticulin in paraffin-embedded Human thyroid cancer using Calreticulin Rabbit polyclonal antibody (STJ11104469) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Calreticulin in paraffin-embedded Human placenta using Calreticulin Rabbit polyclonal antibody (STJ11104469) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Calreticulin in paraffin-embedded Human colon carcinoma using Calreticulin Rabbit polyclonal antibody (STJ11104469) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Calreticulin Rabbit polyclonal antibody (STJ11104469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-CALR antibody (18-417) (STJ11104469)

SKU:
STJ11104469

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Calreticulin (18-417) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CALR
Gene ID: 811
Uniprot ID: CALR_HUMAN
Immunogen Region: 18-417
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 18-417 of human Calreticulineticulin (NP_004334.1).
Immunogen Sequence: PAVYFKEQFLDGDGWTSRWI ESKHKSDFGKFVLSSGKFYG DEEKDKGLQTSQDARFYALS ASFEPFSNKGQTLVVQFTVK HEQNIDCGGGYVKLFPNSLD QTDMHGDSEYNIMFGPDICG PGTKKVHVIFNYKGKNVLIN KDIRCKDDEFTHLYTLIVRP DNTYEVKIDNSQVESGSLED DWDFLPPKKIKDPDASKPED WDERAKIDDPTDSKPEDWDK PEHIPDPDAKKPEDWDEEM
Function Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy.
Protein Name Calreticulin
Crp55
Calregulin
Endoplasmic Reticulum Resident Protein 60
Erp60
Hacbp
Grp60
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-168316
Reactome: R-HSA-3000480
Reactome: R-HSA-3000484
Reactome: R-HSA-381183
Reactome: R-HSA-901042
Reactome: R-HSA-983170
Cellular Localisation Endoplasmic Reticulum Lumen
Cytoplasm
Cytosol
Secreted
Extracellular Space
Extracellular Matrix
Cell Surface
Sarcoplasmic Reticulum Lumen
Cytoplasmic Vesicle
Secretory Vesicle
Cortical Granule
Cytolytic Granule
Also Found In Cell Surface (T Cells)
Cytosol And Extracellular Matrix
During Oocyte Maturation And After Parthenogenetic Activation Accumulates In Cortical Granules
In Pronuclear And Early Cleaved Embryos Localizes Weakly To Cytoplasm Around Nucleus And More Strongly In The Region Near The Cortex
In Cortical Granules Of Non-Activated Oocytes
Is Exocytosed During The Cortical Reaction In Response To Oocyte Activation
Alternative Antibody Names Anti-Calreticulin antibody
Anti-Crp55 antibody
Anti-Calregulin antibody
Anti-Endoplasmic Reticulum Resident Protein 60 antibody
Anti-Erp60 antibody
Anti-Hacbp antibody
Anti-Grp60 antibody
Anti-CALR antibody
Anti-CRTC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance