| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:5000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CALR |
| Gene ID: | 811 |
| Uniprot ID: | CALR_HUMAN |
| Immunogen Region: | 18-203 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 18-203 of human Calreticulineticulin (NP_004334.1). |
| Immunogen Sequence: | EPAVYFKEQFLDGDGWTSRW IESKHKSDFGKFVLSSGKFY GDEEKDKGLQTSQDARFYAL SASFEPFSNKGQTLVVQFTV KHEQNIDCGGGYVKLFPNSL DQTDMHGDSEYNIMFGPDIC GPGTKKVHVIFNYKGKNVLI NKDIRCKDDEFTHLYTLIVR PDNTYEVKIDNSQVESGSLE DDWDFL |
| Function | Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy. |
| Protein Name | Calreticulin Crp55 Calregulin Endoplasmic Reticulum Resident Protein 60 Erp60 Hacbp Grp60 |
| Database Links | Reactome: R-HSA-1236974 Reactome: R-HSA-168316 Reactome: R-HSA-3000480 Reactome: R-HSA-3000484 Reactome: R-HSA-381183 Reactome: R-HSA-901042 Reactome: R-HSA-983170 |
| Cellular Localisation | Endoplasmic Reticulum Lumen Cytoplasm Cytosol Secreted Extracellular Space Extracellular Matrix Cell Surface Sarcoplasmic Reticulum Lumen Cytoplasmic Vesicle Secretory Vesicle Cortical Granule Cytolytic Granule Also Found In Cell Surface (T Cells) Cytosol And Extracellular Matrix During Oocyte Maturation And After Parthenogenetic Activation Accumulates In Cortical Granules In Pronuclear And Early Cleaved Embryos Localizes Weakly To Cytoplasm Around Nucleus And More Strongly In The Region Near The Cortex In Cortical Granules Of Non-Activated Oocytes Is Exocytosed During The Cortical Reaction In Response To Oocyte Activation |
| Alternative Antibody Names | Anti-Calreticulin antibody Anti-Crp55 antibody Anti-Calregulin antibody Anti-Endoplasmic Reticulum Resident Protein 60 antibody Anti-Erp60 antibody Anti-Hacbp antibody Anti-Grp60 antibody Anti-CALR antibody Anti-CRTC antibody |
Information sourced from Uniprot.org