• Western blot analysis of extracts of various cell lines, using CALR antibody (STJ22866) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Calreticulineticulin rabbit polyclonal antibody (STJ22866) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using Calreticulineticulin rabbit polyclonal antibody (STJ22866) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Calreticulineticulin rabbit polyclonal antibody (STJ22866) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Calreticulineticulin rabbit polyclonal antibody (STJ22866) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human brain using Calreticulineticulin rabbit polyclonal antibody (STJ22866) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse pancreas using Calreticulineticulin rabbit polyclonal antibody (STJ22866) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using Calreticulin rabbit polyclonal antibody (STJ22866) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using Calreticulin rabbit polyclonal antibody (STJ22866) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using Calreticulin rabbit polyclonal antibody (STJ22866) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-CALR antibody (18-203) (STJ22866)

SKU:
STJ22866

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CALR (18-203) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CALR
Gene ID: 811
Uniprot ID: CALR_HUMAN
Immunogen Region: 18-203
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 18-203 of human Calreticulineticulin (NP_004334.1).
Immunogen Sequence: EPAVYFKEQFLDGDGWTSRW IESKHKSDFGKFVLSSGKFY GDEEKDKGLQTSQDARFYAL SASFEPFSNKGQTLVVQFTV KHEQNIDCGGGYVKLFPNSL DQTDMHGDSEYNIMFGPDIC GPGTKKVHVIFNYKGKNVLI NKDIRCKDDEFTHLYTLIVR PDNTYEVKIDNSQVESGSLE DDWDFL
Function Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy.
Protein Name Calreticulin
Crp55
Calregulin
Endoplasmic Reticulum Resident Protein 60
Erp60
Hacbp
Grp60
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-168316
Reactome: R-HSA-3000480
Reactome: R-HSA-3000484
Reactome: R-HSA-381183
Reactome: R-HSA-901042
Reactome: R-HSA-983170
Cellular Localisation Endoplasmic Reticulum Lumen
Cytoplasm
Cytosol
Secreted
Extracellular Space
Extracellular Matrix
Cell Surface
Sarcoplasmic Reticulum Lumen
Cytoplasmic Vesicle
Secretory Vesicle
Cortical Granule
Cytolytic Granule
Also Found In Cell Surface (T Cells)
Cytosol And Extracellular Matrix
During Oocyte Maturation And After Parthenogenetic Activation Accumulates In Cortical Granules
In Pronuclear And Early Cleaved Embryos Localizes Weakly To Cytoplasm Around Nucleus And More Strongly In The Region Near The Cortex
In Cortical Granules Of Non-Activated Oocytes
Is Exocytosed During The Cortical Reaction In Response To Oocyte Activation
Alternative Antibody Names Anti-Calreticulin antibody
Anti-Crp55 antibody
Anti-Calregulin antibody
Anti-Endoplasmic Reticulum Resident Protein 60 antibody
Anti-Erp60 antibody
Anti-Hacbp antibody
Anti-Grp60 antibody
Anti-CALR antibody
Anti-CRTC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance