• Western blot analysis of extracts of various cell lines, using CALM2 antibody (STJ110315) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% non-fat dry milk in TBST. _Detection: ECL Basic Kit. _Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using CALM2 antibody (STJ110315) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CALM2 antibody (1-149) (STJ110315)

SKU:
STJ110315

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CALM2
Gene ID: 801/805/808
Uniprot ID: CALM2_HUMAN
Immunogen Region: 1-149
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-149 of human CALM2 (NP_001734.1).
Immunogen Sequence: MADQLTEEQIAEFKEAFSLF DKDGDGTITTKELGTVMRSL GQNPTEAELQDMINEVDADG NGTIDFPEFLTMMARKMKDT DSEEEIREAFRVFDKDGNGY ISAAELRHVMTNLGEKLTDE EVDEMIREADIDGDGQVNYE EFVQMMTAK
Post Translational Modifications Ubiquitination results in a strongly decreased activity. Phosphorylation results in a decreased activity.
Function Calmodulin acts as part of a calcium signal transduction pathway by mediating the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Calcium-binding is required for the activation of calmodulin. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases, such as myosin light-chain kinases and calmodulin-dependent protein kinase type II (CaMK2), and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. Mediates calcium-dependent inactivation of CACNA1C. Positively regulates calcium-activated potassium channel activity of KCNN2. (Microbial infection) Required for C.violaceum CopC and S.flexneri OspC3 arginine ADP-riboxanase activity.
Protein Name Calmodulin-2
Database Links Reactome: R-HSA-111932
Reactome: R-HSA-111933
Reactome: R-HSA-111957
Reactome: R-HSA-111997
Reactome: R-HSA-114608
Reactome: R-HSA-1445148
Reactome: R-HSA-1474151
Reactome: R-HSA-163615
Reactome: R-HSA-180024
Reactome: R-HSA-1855204
Reactome: R-HSA-2025928
Reactome: R-HSA-203615
Reactome: R-HSA-2151201
Reactome: R-HSA-2514859
Reactome: R-HSA-2672351
Reactome: R-HSA-2871809
Reactome: R-HSA-4086398
Reactome: R-HSA-418359
Reactome: R-HSA-425561
Reactome: R-HSA-438066
Reactome: R-HSA-442720
Reactome: R-HSA-442729
Reactome: R-HSA-442982
Reactome: R-HSA-445355
Reactome: R-HSA-451308
Reactome: R-HSA-5210891
Reactome: R-HSA-5218920
Reactome: R-HSA-5218921
Reactome: R-HSA-5576892
Reactome: R-HSA-5578775
Reactome: R-HSA-5607763
Reactome: R-HSA-5626467
Reactome: R-HSA-5627123
Reactome: R-HSA-5673000
Reactome: R-HSA-5673001
Reactome: R-HSA-6802946
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-70221
Reactome: R-HSA-8876725
Reactome: R-HSA-9009391
Reactome: R-HSA-9022535
Reactome: R-HSA-9022692
Reactome: R-HSA-936837
Reactome: R-HSA-9617324
Reactome: R-HSA-9619229
Reactome: R-HSA-9619483
Reactome: R-HSA-9620244
Reactome: R-HSA-9648002
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Reactome: R-HSA-9664323
Reactome: R-HSA-983695
Cellular Localisation Cytoplasm
Cytoskeleton
Spindle
Spindle Pole
Microtubule Organizing Center
Centrosome
Distributed Throughout The Cell During Interphase
But During Mitosis Becomes Dramatically Localized To The Spindle Poles And The Spindle Microtubules
Alternative Antibody Names Anti-Calmodulin-2 antibody
Anti-CALM2 antibody
Anti-CAM2 antibody
Anti-CAMB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance