| Host: |
Rabbit |
| Applications: |
IHC/IF/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CAD protein (422-471 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CAD |
| Gene ID: |
790 |
| Uniprot ID: |
PYR1_HUMAN |
| Immunogen Region: |
422-471 aa |
| Specificity: |
CAD Polyclonal Antibody detects endogenous levels of CAD protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human CAD at the amino acid range 422-471 |
| Function | Multifunctional protein that encodes the first 3 enzymatic activities of the de novo pyrimidine pathway: carbamoylphosphate synthetase (CPSase.EC 6.3.5.5), aspartate transcarbamylase (ATCase.EC 2.1.3.2) and dihydroorotase (DHOase.EC 3.5.2.3). The CPSase-function is accomplished in 2 steps, by a glutamine-dependent amidotransferase activity (GATase) that binds and cleaves glutamine to produce ammonia, followed by an ammonium-dependent carbamoyl phosphate synthetase, which reacts with the ammonia, hydrogencarbonate and ATP to form carbamoyl phosphate. The endogenously produced carbamoyl phosphate is sequestered and channeled to the ATCase active site. ATCase then catalyzes the formation of carbamoyl-L-aspartate from L-aspartate and carbamoyl phosphate. In the last step, DHOase catalyzes the cyclization of carbamoyl aspartate to dihydroorotate. |
| Protein Name | Multifunctional Protein CadCarbamoyl Phosphate Synthetase 2-Aspartate Transcarbamylase-Dihydroorotase Includes - Glutamine-Dependent Carbamoyl Phosphate Synthase - Glutamine AmidotransferaseGataseGlnase - Ammonium-Dependent Carbamoyl Phosphate SynthaseCpsCpsase - Aspartate Carbamoyltransferase - Dihydroorotase |
| Database Links | Reactome: R-HSA-500753 |
| Cellular Localisation | CytoplasmNucleusCytosolic And Unphosphorylated In Resting CellsTranslocates To The Nucleus In Response To Egf StimulationNuclear Import Promotes Optimal Cell Growth |
| Alternative Antibody Names | Anti-Multifunctional Protein Cad antibodyAnti-Carbamoyl Phosphate Synthetase 2-Aspartate Transcarbamylase-Dihydroorotase Includes - Glutamine-Dependent Carbamoyl Phosphate Synthase - Glutamine Amidotransferase antibodyAnti-Gatase antibodyAnti-Glnase - Ammonium-Dependent Carbamoyl Phosphate Synthase antibodyAnti-Cps antibodyAnti-Cpsase - Aspartate Carbamoyltransferase - Dihydroorotase antibodyAnti-CAD antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
