| Short Description : | Mouse monoclonal anti-Calcyclin-binding protein for use in WB, IHC, IF and ELISA in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC/IF/ELISA |
| Host: | Mouse |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | 7D11 |
| Isotype: | IgG1 |
| Conjugation: | Unconjugated |
| Concentration: | 1 mg/mL |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000IHC 1:200-1:1000ELISA 1:10000IF 1:50-200 |
| Formulation: | Liquid in PBS containing 0.03% Sodium Azide. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CACYBP |
| Gene ID: | 27101 |
| Uniprot ID: | CYBP_HUMAN |
| Immunogen: | Purified recombinant fragment of Calcyclin expressed in E. Coli. |
| Specificity: | Calcyclin Monoclonal Antibody detects endogenous levels of Calcyclin protein. |
| Function | May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1). |
| Protein Name | Calcyclin-Binding ProteinCacybpHcacybpS100a6-Binding ProteinSiah-Interacting Protein |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmCytoplasmic At Low Calcium ConcentrationsIn Neuroblastoma CellsAfter A Retinoic Acid (Ra) Induction And Calcium IncreaseIt Localizes In Both The Nucleus And CytoplasmThe Nuclear Fraction May Be Phosphorylated |
| Alternative Antibody Names | Anti-Calcyclin-Binding Protein antibodyAnti-Cacybp antibodyAnti-Hcacybp antibodyAnti-S100a6-Binding Protein antibodyAnti-Siah-Interacting Protein antibodyAnti-CACYBP antibodyAnti-S100A6BP antibodyAnti-SIP antibodyAnti-PNAS-107 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and CACYBP knockout (KO) 293T cells, using [KO Validated] CACYBP Rabbit polyclonal antibody (STJ11100880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89814/358277/STJ11100880_1__41081.1713122624.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CACYBP knockout (KO) 293T cells, using [KO Validated] CACYBP Rabbit polyclonal antibody (STJ11100880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of various lysates using [KO Validated] CACYBP Rabbit pAb (STJ111403) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/91786/408693/STJ111403_1__96822.1744895032.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] CACYBP Rabbit pAb (STJ111403) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.")
