![Western blot analysis of lysates from wild type (WT) and CACYBP knockout (KO) 293T cells, using [KO Validated] CACYBP Rabbit polyclonal antibody (STJ11100880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89814/358277/STJ11100880_1__41081.1713122624.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CACYBP knockout (KO) 293T cells, using [KO Validated] CACYBP Rabbit polyclonal antibody (STJ11100880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and CACYBP knockout (KO) 293T cells, using [KO Validated] CACYBP Rabbit polyclonal antibody (STJ11100880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89814/358277/STJ11100880_1__41081.1713122624.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CACYBP knockout (KO) 293T cells, using [KO Validated] CACYBP Rabbit polyclonal antibody (STJ11100880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:1000-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CACYBP |
| Gene ID: | 27101 |
| Uniprot ID: | CYBP_HUMAN |
| Immunogen Region: | 1-228 |
| Immunogen Sequence: | MASEELQKDLEEVKVLLEKA TRKRVRDALTAEKSKIETEI KNKMQQKSQKKAELLDNEKP AAVVAPITTGYTVKISNYGW DQSDKFVKIYITLTGVHQVP TENVQVHFTERSFDLLVKNL NGKSYSMIVNNLLKPISVEG SSKKVKTDTVLILCRKKVEN TRWDYLTQVEKECKEKEKPS YDTETDPSEGLMNVLKKIYE DGDDDMKRTINKAWVESREK QAKGDTEF |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-228 of human CACYBP (NP_055227.1). |
| Post Translational Modifications | Phosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations. |
| Function | May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1). |
| Protein Name | Calcyclin-Binding ProteinCacybpHcacybpS100a6-Binding ProteinSiah-Interacting Protein |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmCytoplasmic At Low Calcium ConcentrationsIn Neuroblastoma CellsAfter A Retinoic Acid (Ra) Induction And Calcium IncreaseIt Localizes In Both The Nucleus And CytoplasmThe Nuclear Fraction May Be Phosphorylated |
| Alternative Antibody Names | Anti-Calcyclin-Binding Protein antibodyAnti-Cacybp antibodyAnti-Hcacybp antibodyAnti-S100a6-Binding Protein antibodyAnti-Siah-Interacting Protein antibodyAnti-CACYBP antibodyAnti-S100A6BP antibodyAnti-SIP antibodyAnti-PNAS-107 antibody |
Information sourced from Uniprot.org
![Western blot analysis of various lysates using [KO Validated] CACYBP Rabbit pAb (STJ111403) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/91786/408693/STJ111403_1__96822.1744895032.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] CACYBP Rabbit pAb (STJ111403) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.")
