• Immunofluorescence analysis of HeLa cells using Carbonic Anhydrase 9 (CA9/G250) antibody (STJ115637) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human gastric cancer using Carbonic Anhydrase 9 (CA9/G250) antibody (STJ115637) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using Carbonic Anhydrase 9 (CA9/G250) antibody (STJ115637) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Carbonic Anhydrase 9 (CA9/G250) Antibody (STJ115637) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-CA9 antibody (52-151) (STJ115637)

SKU:
STJ115637

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CA9 (52-151) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CA9
Gene ID: 768
Uniprot ID: CAH9_HUMAN
Immunogen Region: 52-151
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 52-151 of human Carbonic Anhydrase 9 (CA9/G250) (NP_001207.2).
Immunogen Sequence: GSSGEDDPLGEEDLPSEEDS PREEDPPGEEDLPGEEDLPG EEDLPEVKPKSEEEGSLKLE DLPTVEAPGDPQEPQNNAHR DKEGDDQSHWRYGGDPPWPR
Tissue Specificity Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
Post Translational Modifications Asn-346 bears high-mannose type glycan structures.
Function Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).
Protein Name Carbonic Anhydrase 9
Carbonate Dehydratase Ix
Carbonic Anhydrase Ix
Ca-Ix
Caix
Membrane Antigen Mn
P54/58n
Renal Cell Carcinoma-Associated Antigen G250
Rcc-Associated Antigen G250
Pmw1
Database Links Reactome: R-HSA-1234158
Reactome: R-HSA-1475029
Cellular Localisation Nucleus
Nucleolus
Cell Membrane
Single-Pass Type I Membrane Protein
Cell Projection
Microvillus Membrane
Found On The Surface Microvilli And In The Nucleus
Particularly In Nucleolus
Alternative Antibody Names Anti-Carbonic Anhydrase 9 antibody
Anti-Carbonate Dehydratase Ix antibody
Anti-Carbonic Anhydrase Ix antibody
Anti-Ca-Ix antibody
Anti-Caix antibody
Anti-Membrane Antigen Mn antibody
Anti-P54/58n antibody
Anti-Renal Cell Carcinoma-Associated Antigen G250 antibody
Anti-Rcc-Associated Antigen G250 antibody
Anti-Pmw1 antibody
Anti-CA9 antibody
Anti-G250 antibody
Anti-MN antibody

Information sourced from Uniprot.org

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