• Western blot analysis of extracts of various cell lines, using C7orf50 antibody (STJ116694) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-C7orf50 antibody (1-194) (STJ116694)

SKU:
STJ116694

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: C7orf50
Gene ID: 84310
Uniprot ID: CHOLN_HUMAN
Immunogen Region: 1-194
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human C7orf50 (NP_115726.1).
Immunogen Sequence: MAKQKRKVPEVTEKKNKKLK KASAEGPLLGPEAAPSGEGA GSKGEAVLRPGLDAEPELSP EEQRVLERKLKKERKKEERQ RLREAGLVAQHPPARRSGAE LALDYLCRWAQKHKNWRFQK TRQTWLLLHMYDSDKVPDEH FSTLLAYLEGLQGRARELTV QKAEALMRELDEEGSDPPLP GRAQRIRQVLQLLS
Tissue Specificity Secreted from the instestine, secretion is induced by feeding and cholesterol absorption.
Function Hormone secreted from the intestine in response to cholesterol, where it acts to inhibit cholesterol synthesis in the liver and VLDL secretion,leading to a reduction in circulating cholesterol levels. Acts through binding to its receptor, GPR146.
Protein Name Protein Cholesin
Cellular Localisation Secreted
Secreted Via Exosomes
Secretion Is Induced By Feeding And Cholesterol Absorption
Alternative Antibody Names Anti-Protein Cholesin antibody
Anti-C7orf50 antibody
Anti-FP15621 antibody

Information sourced from Uniprot.org

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