| Host: | Rabbit |
| Applications: | IF/ICC/ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | SRM |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | IF/ICC:1:200-1:800ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: | C3AR1 |
| Gene ID: | 719 |
| Uniprot ID: | C3AR_HUMAN |
| Immunogen Region: | 1-100 aa |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human C3AR1 (NP_004045.1). |
| Immunogen Sequence: | MASFSAETNSTDLLSQPWNE PPVILSMVILSLTFLLGLPG NGLVLWVAGLKMQRTVNTIW FLHLTLADLLCCLSLPFSLA HLALQGQWPYGRFLCKLIPS |
| Tissue Specificity | Widely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues. |
| Post Translational Modifications | Among the sulfation sites Tyr-174 is essential for binding of C3a anaphylatoxin. O-glycosylated. |
| Function | Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. |
| Protein Name | C3a Anaphylatoxin Chemotactic ReceptorC3arC3a-R |
| Database Links | Reactome: R-HSA-375276Reactome: R-HSA-418594Reactome: R-HSA-6798695Reactome: R-HSA-9660826Reactome: R-HSA-977606 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-C3a Anaphylatoxin Chemotactic Receptor antibodyAnti-C3ar antibodyAnti-C3a-R antibodyAnti-C3AR1 antibodyAnti-AZ3B antibodyAnti-C3R1 antibodyAnti-HNFAG09 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from SH-SY5Y cells using [KD Validated] ETV1 Rabbit mAb (STJ11107174) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 45s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/186370/408075/STJ11107174_1__85519.1744894498.jpg?c=1 "Western blot analysis of lysates from SH-SY5Y cells using [KD Validated] ETV1 Rabbit mAb (STJ11107174) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 45s.")
