• Immunofluorescence analysis of L929 cells using C18orf8 Rabbit polyclonal antibody (STJ118270) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of C18orf8 in paraffin-embedded Human colon carcinoma using C18orf8 Rabbit polyclonal antibody (STJ118270) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of C18orf8 in paraffin-embedded Rat kidney using C18orf8 Rabbit polyclonal antibody (STJ118270) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of C18orf8 in paraffin-embedded Mouse kidney using C18orf8 Rabbit polyclonal antibody (STJ118270) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using C18orf8 Rabbit polyclonal antibody (STJ118270) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-C18orf8 antibody (1-300) (STJ118270)

SKU:
STJ118270

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-C18orf8 (1-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RMC1
Gene ID: 29919
Uniprot ID: RMC1_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human C18orf8 (NP_037458.3).
Immunogen Sequence: MGEEDYYLELCERPVQFEKA NPVNCVFFDEANKQVFAVRS GGATGVVVKGPDDRNPISFR MDDKGEVKCIKFSLENKILA VQRTSKTVDFCNFIPDNSQL EYTQECKTKNANILGFCWTS STEIVFITDQGIEFYQVLPE KRSLKLLKSHNLNVNWYMYC PESAVILLSTTVLENVLQPF HFRAGTMSKLPKFEIELPAA PKSTKPSLSERDIAMATIYG QLYVLFLRHHSRTSNSTGA
Function Componement of the CCZ1-MON1 RAB7A guanine exchange factor (GEF). Acts as a positive regulator of CCZ1-MON1A/B function necessary for endosomal/autophagic flux and efficient RAB7A localization.
Protein Name Regulator Of Mon1-Ccz1 Complex
Colon Cancer-Associated Protein Mic1
Mic-1
Wd Repeat-Containing Protein 98
Cellular Localisation Lysosome Membrane
Late Endosome Membrane
Alternative Antibody Names Anti-Regulator Of Mon1-Ccz1 Complex antibody
Anti-Colon Cancer-Associated Protein Mic1 antibody
Anti-Mic-1 antibody
Anti-Wd Repeat-Containing Protein 98 antibody
Anti-RMC1 antibody
Anti-C18orf8 antibody
Anti-MIC1 antibody
Anti-WDR98 antibody

Information sourced from Uniprot.org

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