• Western blot analysis of extracts of mouse testis, using C16orf80 antibody (STJ29179) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of Rat lung, using C16orf80 antibody (STJ29179) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of SKOV3 cells, using C16orf80 antibody (STJ29179) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of A549 cells using C16orf80 antibody (STJ29179). Blue: DAPI for nuclear staining.

Anti-C16orf80 antibody (94-193) (STJ29179)

SKU:
STJ29179

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CFAP20
Gene ID: 29105
Uniprot ID: CFA20_HUMAN
Immunogen Region: 94-193
Specificity: A synthetic peptide corresponding to a sequence within amino acids 94-193 of human C16orf80 (NP_037374.1).
Immunogen Sequence: LDDKNVRRRFRASNYQSTTR VKPFICTMPMRLDDGWNQIQ FNLLDFTRRAYGTNYIETLR VQIHANCRIRRVYFSDRLYS EDELPAEFKLYLPVQNKAKQ
Function Cilium- and flagellum-specific protein that plays a role in axonemal structure organization and motility. Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating. Involved in the regulation of the size and morphology of cilia. Required for axonemal microtubules polyglutamylation.
Protein Name Cilia- And Flagella-Associated Protein 20
Basal Body Up-Regulated Protein 22
Transcription Factor Iib
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Cilium Basal Body
Cilium Axoneme
Flagellum Axoneme
Alternative Antibody Names Anti-Cilia- And Flagella-Associated Protein 20 antibody
Anti-Basal Body Up-Regulated Protein 22 antibody
Anti-Transcription Factor Iib antibody
Anti-CFAP20 antibody
Anti-BUG22 antibody
Anti-C16orf80 antibody
Anti-GTL3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance