| Host: |
Goat |
| Applications: |
Pep-ELISA/WB |
| Reactivity: |
Human/Mouse/Rat/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-C14orf169/NO66 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 70kDa band observed in nuclear lysates of cell lines A431, HepG2 and NIH3T3 (calculated MW of 71.1kDa according to Human NP_078920.2 and 67.6kDa according to Mouse NP_076122.2) |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
RIOX1 |
| Gene ID: |
79697 |
| Uniprot ID: |
RIOX1_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_078920.2 |
| Immunogen Sequence: |
GAQHSDSKDPR |
| Function | Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation. Also catalyzes demethylation of non-histone proteins, such as CGAS: demethylation of monomethylated CGAS promotes interaction between CGAS and PARP1, followed by PARP1 inactivation. Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216', thereby playing a role in ribosome biogenesis. Participates in MYC-induced transcriptional activation. |
| Protein Name | Ribosomal Oxygenase 160s Ribosomal Protein L8 Histidine HydroxylaseBifunctional Lysine-Specific Demethylase And Histidyl-Hydroxylase No66Myc-Associated Protein With Jmjc DomainNucleolar Protein 66Hsno66Ribosomal Oxygenase No66Rox |
| Database Links | Reactome: R-HSA-9629569 |
| Cellular Localisation | NucleusNucleolusNucleoplasmGranular Part Of NucleoliNucleoplasmic FociSome Of Them Associated With Nucleoli |
| Alternative Antibody Names | Anti-Ribosomal Oxygenase 1 antibodyAnti-60s Ribosomal Protein L8 Histidine Hydroxylase antibodyAnti-Bifunctional Lysine-Specific Demethylase And Histidyl-Hydroxylase No66 antibodyAnti-Myc-Associated Protein With Jmjc Domain antibodyAnti-Nucleolar Protein 66 antibodyAnti-Hsno66 antibodyAnti-Ribosomal Oxygenase No66 antibodyAnti-Rox antibodyAnti-RIOX1 antibodyAnti-C14orf169 antibodyAnti-MAPJD antibodyAnti-NO66 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
