• Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa c-FOS protein.
  • Immunofluorescence of a section of rat hippocampus showing neurons labeled with c-FOS (STJA0003594, 1:1000, red) counterstained with FOX3 (NeuN) (green) , and nuclear staining with DAPI. When both c-FOS and FOX3 are expressed the label appears orange.
  • Immunostaining of mouse brain labeling c-Fos protein in green (STJA0003594, 1:1000) and DNA with DAPI. Photo courtesy of Adam Almeida, University of Colorado.
  • Immunolabeling of a section of rat brain that specifically labels c-FOS protein (STJA0003594, green, 1:1000). Frozen sections were fixed with 4%PFA for 24 hours. Before performing free floating immunohistochemistry, sections underwent antigen retrieval with sodium citrate (pH6. 0) for 30 minutes at 80˚C. Image kindly provided by Angela Gonzalez, Washington State University, Vancouver.
  • Immunofluorescence of serum starved, FBS stimulated HeLa cells showing nuclear labeling of activated cells with Anti-c-FOS (STJA0003594, 1:1000, green) while Anti-Vimentin (STJA0003823, 1:500, red) labels the cytoplasmic intermediate filament, and nuclear staining with DAPI (blue).

Anti-c-FOS antibody (Full Length) [2H2] (STJA0003594)

SKU:
STJA0003594-100

£442.50
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Host: Mouse
Applications: WB/IHC/ICC
Reactivity: Bovine/Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-c-FOS (Full Length) is suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: 2H2
Conjugation: Unconjugated
Isotype: IgG1
Formulation: 100 ul in PBS + 50% Glycerol and 5 mM Sodium Azide
Purification: This antibody was protein g purified culture from supernatant.
Dilution Range: WB 1:2000
IHC 1:1000
ICC 1:1000
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FOS
Gene ID: 2353
Uniprot ID: FOS_HUMAN
Immunogen Region: Full Length
Immunogen: Recombinant full length human c-FOS expressed in and purification from E. Coli.
Post Translational Modifications Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation. Constitutively sumoylated with SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232. In quiescent cells, the small amount of FOS present is phosphorylated at Tyr-10 and Tyr-30 by SRC. This Tyr-phosphorylated form is cytosolic. In growing cells, dephosphorylated by PTPN2. Dephosphorylation leads to the association with endoplasmic reticulum membranes and activation of phospholipid synthesis.
Function Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
Protein Name Protein C-Fos
Cellular Oncogene Fos
Fos Proto-Oncogene - Ap-1 Transcription Factor Subunit
G0/G1 Switch Regulatory Protein 7
Proto-Oncogene C-Fos
Transcription Factor Ap-1 Subunit C-Fos
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2871796
Reactome: R-HSA-450341
Reactome: R-HSA-6785807
Reactome: R-HSA-6796648
Reactome: R-HSA-9018519
Reactome: R-HSA-9031628
Reactome: R-HSA-9634638
Reactome: R-HSA-9768919
Cellular Localisation Nucleus
Endoplasmic Reticulum
Cytoplasm
Cytosol
In Quiescent Cells
Present In Very Small Amounts In The Cytosol
Following Induction Of Cell Growth
First Localizes To The Endoplasmic Reticulum And Only Later To The Nucleus
Localization At The Endoplasmic Reticulum Requires Dephosphorylation At Tyr-10 And Tyr-30
Alternative Antibody Names Anti-Protein C-Fos antibody
Anti-Cellular Oncogene Fos antibody
Anti-Fos Proto-Oncogene - Ap-1 Transcription Factor Subunit antibody
Anti-G0/G1 Switch Regulatory Protein 7 antibody
Anti-Proto-Oncogene C-Fos antibody
Anti-Transcription Factor Ap-1 Subunit C-Fos antibody
Anti-FOS antibody
Anti-G0S7 antibody

Information sourced from Uniprot.org

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