| Host: | Rabbit |
| Applications: | WB/IHC-P/ICC/IF/IP/FC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit monoclonal anti-BUB3 for use in WB, IHC-P, ICC, IF, IP and FC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | R01-4W1 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification: | Affinity Chromatography |
| Dilution Range: | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:50FC 1:50-1:100 |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: | BUB3 |
| Gene ID: | 9184 |
| Uniprot ID: | BUB3_HUMAN |
| Immunogen: | A synthesized peptide derived from human Bub3 |
| Post Translational Modifications | Poly-ADP-ribosylated by PARP1. Ubiquitinated by UBR5, promoting disassembly of the mitotic checkpoint complex from the APC/C complex. |
| Function | Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. |
| Protein Name | Mitotic Checkpoint Protein Bub3 |
| Database Links | Reactome: R-HSA-141430Reactome: R-HSA-141444Reactome: R-HSA-174184Reactome: R-HSA-176409Reactome: R-HSA-179409Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreStarts To Localize At Kinetochores In Prometaphase I (Pro-Mi) Stage And Maintains The Localization Until The Metaphase I-Anaphase I (Mi-Ai) Transition |
| Alternative Antibody Names | Anti-Mitotic Checkpoint Protein Bub3 antibodyAnti-BUB3 antibody |
Information sourced from Uniprot.org
