• Immunofluorescence analysis of SH-SY5Y cells using BSCL2 Rabbit polyclonal antibody (STJ116793) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of BSCL2 in paraffin-embedded human breast cancer using BSCL2 Rabbit polyclonal antibody (STJ116793) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using BSCL2 Rabbit polyclonal antibody (STJ116793) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BSCL2 antibody (265-363) (STJ116793)

SKU:
STJ116793

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BSCL2 (265-363) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BSCL2
Gene ID: 26580
Uniprot ID: BSCL2_HUMAN
Immunogen Region: 265-363
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 265-363 of human BSCL2 (NP_116056.3).
Immunogen Sequence: RHRFSLQVNIRKRDNSRKEV QRRISAHQPGPEGQEESTPQ SDVTEDGESPEDPSGTEGQL SEEEKPDQQPLSGEEELEPE ASDGSGSWEDAALLTEANL
Tissue Specificity Expressed in motor neurons in the spinal cord and cortical neurons in the frontal lobe (at protein level). Highly expressed in brain, testis and adipose tissue.
Function Plays a crucial role in the formation of lipid droplets (LDs) which are storage organelles at the center of lipid and energy homeostasis. In association with LDAF1, defines the sites of LD formation in the ER. Also required for growth and maturation of small nascent LDs into larger mature LDs. Mediates the formation and/or stabilization of endoplasmic reticulum-lipid droplets (ER-LD) contacts, facilitating protein and lipid delivery from the ER into growing LDs. Regulates the maturation of ZFYVE1-positive nascent LDs and the function of the RAB18-ZFYVE1 complex in mediating the formation of ER-LD contacts. Binds anionic phospholipids including phosphatidic acid. Plays an important role in the differentiation and development of adipocytes.
Protein Name Seipin
Bernardinelli-Seip Congenital Lipodystrophy Type 2 Protein
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Lipid Droplet
Localizes At Endoplasmic Reticulum-Lipid Droplets (Er-Ld) Contact Sites
Alternative Antibody Names Anti-Seipin antibody
Anti-Bernardinelli-Seip Congenital Lipodystrophy Type 2 Protein antibody
Anti-BSCL2 antibody

Information sourced from Uniprot.org

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