• Western blot analysis of extracts of various cell lines, using BRF2 antibody (STJ116330) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of various lysates using BRF2 Rabbit polyclonal antibody (STJ116330) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-BRF2 antibody (180-419) (STJ116330)

SKU:
STJ116330

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BRF2 (180-419) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BRF2
Gene ID: 55290
Uniprot ID: BRF2_HUMAN
Immunogen Region: 180-419
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 180-419 of human BRF2 (NP_060780.2).
Immunogen Sequence: FKLFQASPSVPAKYVEDKEK MLSRTMQLVELANETWLVTG RHPLPVITAATFLAWQSLQP ADRLSCSLARFCKLANVDLP YPASSRLQELLAVLLRMAEQ LAWLRVLRLDKRSVVKHIGD LLQHRQSLVRSAFRDGTAEV ETREKEPPGWGQGQGEGEVG NNSLGLPQGKRPASPALLLP PCMLKSPKRICPVPPVSTVT GDENISDSEIEQYLRTPQEV RDFQRAQAARQAATSVPNP
Post Translational Modifications In response to oxidative stress, Cys-361 is reversibly oxidized to cysteine sulfenic acid. Oxidation of Cys-361 impairs formation of a ternary complex with TBP and DNA and down-regulates expression of target genes in response to oxidative stress.
Function General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. Contributes to the regulation of gene expression.functions as activator in the absence of oxidative stress. Down-regulates expression of target genes in response to oxidative stress. Overexpression protects cells against apoptosis in response to oxidative stress.
Protein Name Transcription Factor Iiib 50 Kda Subunit
Tfiiib50
Htfiiib50
B-Related Factor 2
Brf-2
Hbrfu
Database Links Reactome: R-HSA-749476
Reactome: R-HSA-76071
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Iiib 50 Kda Subunit antibody
Anti-Tfiiib50 antibody
Anti-Htfiiib50 antibody
Anti-B-Related Factor 2 antibody
Anti-Brf-2 antibody
Anti-Hbrfu antibody
Anti-BRF2 antibody
Anti-BRFU antibody
Anti-PRO1470 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance