Host: | Mouse |
Applications: | WB/IHC/IF/ELISA |
Reactivity: | Human |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Mouse monoclonal antibody anti-Serine/threonine-protein kinase B-raf is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
Clonality: | Monoclonal |
Clone ID: | 1H12F1/1H12G10/1F12F11C9 |
Conjugation: | Unconjugated |
Isotype: | IgG1 |
Formulation: | Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
Purification: | Affinity purification |
Dilution Range: | WB 1:500-1:2000 IHC 1:200-1:1000 ELISA 1:10000 IF 1:50-200 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | BRAF |
Gene ID: | 673 |
Uniprot ID: | BRAF_HUMAN |
Specificity: | Raf-B Monoclonal Antibody detects endogenous levels of Raf-B protein. |
Immunogen: | Purified recombinant fragment of Raf-B expressed in E. Coli. |
Post Translational Modifications | Phosphorylation at Ser-365 by SGK1 inhibits its activity. Methylation at Arg-671 decreases stability and kinase activity. Ubiquitinated by RNF149.which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF. |
Function | Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. Phosphorylates PFKFB2. May play a role in the postsynaptic responses of hippocampal neurons. |
Protein Name | Serine/Threonine-Protein Kinase B-Raf Proto-Oncogene B-Raf P94 V-Raf Murine Sarcoma Viral Oncogene Homolog B1 |
Database Links | Reactome: R-HSA-1295596 Reactome: R-HSA-170968 Reactome: R-HSA-170984 Reactome: R-HSA-187706 Reactome: R-HSA-5673000 Reactome: R-HSA-5674135 Reactome: R-HSA-5674499 Reactome: R-HSA-5675221 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802955 Reactome: R-HSA-9649948 Reactome: R-HSA-9656223 Reactome: R-HSA-9726840 Reactome: R-HSA-9726842 |
Cellular Localisation | Nucleus Cytoplasm Cell Membrane Colocalizes With Rgs14 And Raf1 In Both The Cytoplasm And Membranes |
Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase B-Raf antibody Anti-Proto-Oncogene B-Raf antibody Anti-P94 antibody Anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 antibody Anti-BRAF antibody Anti-BRAF1 antibody Anti-RAFB1 antibody |
Information sourced from Uniprot.org