Anti-BNIP1 antibody (50-150) [S4MR] (STJ11103564)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal BNIP1 (50-150) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S4MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BNIP1 |
| Gene ID | 662 |
| Uniprot ID | SEC20_HUMAN |
| Immunogen Region | 50-150 |
| Immunogen Sequence | KFQQLRHRIQDLEQLAKEQD KESEKQLLLQEVENHKKQML SNQASWRKANLTCKIAIDNL EKAELLQGGDLLRQRKTTKE SLAQTSSTITESLMGISRMM A |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human BNIP1 (Q12981). |
Additional Info
| Tissue Specificity | Isoform 1 is highly expressed in heart, brain, liver skeletal muscle and pancreas. Isoform 3 is moderately expressed in placenta, lung and kidney. Isoform 4 is highly expressed in testis and small intestine. |
| Post Translational Modifications | Polyubiquitinated. 'Lys-63'-linked polyubiquitination by RNF185 increases the interaction with the autophagy receptor SQSTM1. Undergoes 'Lys-29'- and 'Lys-63'-linked polyubiquitination by RNF186 that may regulate BNIP1 localization to the mitochondrion. |
| Function | As part of a SNARE complex may be involved in endoplasmic reticulum membranes fusion and be required for the maintenance of endoplasmic reticulum organization. Also plays a role in apoptosis. It is for instance required for endoplasmic reticulum stress-induced apoptosis. As a substrate of RNF185 interacting with SQSTM1, might also be involved in mitochondrial autophagy (Probable). |
| Protein Name | Vesicle Transport Protein Sec20Bcl2/Adenovirus E1b 19 Kda Protein-Interacting Protein 1Transformation-Related Gene 8 ProteinTrg-8 |
| Database Links | Reactome: R-HSA-6811434 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type Iv Membrane ProteinMitochondrion MembraneLocalization To The Mitochondrion Is Regulated By Rnf186 |
| Alternative Antibody Names | Anti-Vesicle Transport Protein Sec20 antibodyAnti-Bcl2/Adenovirus E1b 19 Kda Protein-Interacting Protein 1 antibodyAnti-Transformation-Related Gene 8 Protein antibodyAnti-Trg-8 antibodyAnti-BNIP1 antibodyAnti-NIP1 antibodyAnti-SEC20L antibodyAnti-TRG8 antibody |
Information sourced from Uniprot.org