• Immunohistochemistry analysis of paraffin-embedded mouse heart using BMP3 antibody (STJ28957) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using BMP3 antibody (STJ28957) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using BMP3 antibody (STJ28957) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using BMP3 antibody (STJ28957) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using BMP3 antibody (STJ28957) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-BMP3 antibody (243-472) (STJ28957)

SKU:
STJ28957

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BMP3 (243-472) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BMP3
Gene ID: 651
Uniprot ID: BMP3_HUMAN
Immunogen Region: 243-472
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 243-472 of human BMP3 (NP_001192.2).
Immunogen Sequence: YANDAAISEPESVVSSLQGH RNFPTGTVPKWDSHIRAALS IERRKKRSTGVLLPLQNNEL PGAEYQYKKDEVWEERKPYK TLQAQAPEKSKNKKKQRKGP HRKSQTLQFDEQTLKKARRK QWIEPRNCARRYLKVDFADI GWSEWIISPKSFDAYYCSGA CQFPMPKSLKPSNHATIQSI VRAVGVVPGIPEPCCVPEKM SSLSILFFDENKNVVLKVYP NMTVESCACR
Tissue Specificity Expressed in adult and fetal cartilage.
Function Growth factor of the TGF-beta superfamily that plays an essential role in developmental process by inducing and patterning early skeletal formation and by negatively regulating bone density. Antagonizes the ability of certain osteogenic BMPs to induce osteoprogenitor differentiation and ossification. Initiates signaling cascades by associating with type II receptor ACVR2B to activate SMAD2-dependent and SMAD-independent signaling cascades including TAK1 and JNK pathways.
Protein Name Bone Morphogenetic Protein 3
Bmp-3
Bone Morphogenetic Protein 3a
Bmp-3a
Osteogenin
Cellular Localisation Secreted
Alternative Antibody Names Anti-Bone Morphogenetic Protein 3 antibody
Anti-Bmp-3 antibody
Anti-Bone Morphogenetic Protein 3a antibody
Anti-Bmp-3a antibody
Anti-Osteogenin antibody
Anti-BMP3 antibody
Anti-BMP3A antibody

Information sourced from Uniprot.org

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