• Immunofluorescence analysis of U2OS cells using BLZF1 antibody (STJ28513). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using BLZF1 antibody (STJ28513) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BLZF1 antibody (1-400) (STJ28513)

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STJ28513

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BLZF1 (1-400) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BLZF1
Gene ID: 8548
Uniprot ID: GO45_HUMAN
Immunogen Region: 1-400
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-400 of human BLZF1 (NP_003657.1).
Immunogen Sequence: MTTKNLETKVTVTSSPIRGA GDGMETEEPPKSVEVTSGVQ SRKHHSLQSPWKKAVPSESP GVLQLGKMLTEKAMEVKAVR ILVPKAAITHDIPNKNTKVK SLGHHKGEFLGQSEGVIEPN KELSEVKNVLEKLKNSERRL LQDKEGLSNQLRVQTEVNRE LKKLLVASVGDDLQYHFERL AREKNQLILENEALGRNTAQ LSEQLERMSIQCDVWRSKFL ASRVMADELTNSRAALQRQ
Tissue Specificity Detected in adrenal gland.
Post Translational Modifications ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination. Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation.
Function Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.
Protein Name Golgin-45
Basic Leucine Zipper Nuclear Factor 1
Jem-1
P45 Basic Leucine-Zipper Nuclear Factor
Database Links Reactome: R-HSA-162658
Cellular Localisation Golgi Apparatus Membrane
Isoform 1: Nucleus
Detected In The Nucleus Upon Heterologous Expression
Not Detected In The Cytoplasm
Isoform 2: Cytoplasm
Not Detected In The Nucleus
Alternative Antibody Names Anti-Golgin-45 antibody
Anti-Basic Leucine Zipper Nuclear Factor 1 antibody
Anti-Jem-1 antibody
Anti-P45 Basic Leucine-Zipper Nuclear Factor antibody
Anti-BLZF1 antibody
Anti-JEM1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance