• STJ73391 (0. 3µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-BLVRB antibody (C-Term) (STJ73391)

SKU:
STJ73391-100

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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-BLVRB (C-Term) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 23kDa band observed in Human Liver lysates (calculated MW of 22.1kDa according to NP_000704.1). Recommended concentration: 0.3-1µg/ml.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: BLVRB
Gene ID: 645
Uniprot ID: BLVRB_HUMAN
Immunogen Region: C-Term
Accession Number: NP_000704.1
Immunogen Sequence: TTDEYDGHSTYP
Function Enzyme that can both act as a NAD(P)H-dependent reductase and a S-nitroso-CoA-dependent nitrosyltransferase. Promotes fetal heme degradation during development. Also expressed in adult tissues, where it acts as a regulator of hematopoiesis, intermediary metabolism (glutaminolysis, glycolysis, TCA cycle and pentose phosphate pathway) and insulin signaling. Has a broad specificity oxidoreductase activity by catalyzing the NAD(P)H-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone). Contributes to fetal heme catabolism by catalyzing reduction of biliverdin IXbeta into bilirubin IXbeta in the liver. Biliverdin IXbeta, which constitutes the major heme catabolite in the fetus is not present in adult. Does not reduce bilirubin IXalpha. Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH. Acts as a protein nitrosyltransferase by catalyzing nitrosylation of cysteine residues of target proteins, such as HMOX2, INSR and IRS1. S-nitroso-CoA-dependent nitrosyltransferase activity is mediated via a 'ping-pong' mechanism: BLVRB first associates with both S-nitroso-CoA and protein substrate, nitric oxide group is then transferred from S-nitroso-CoA to Cys-109 and Cys-188 residues of BLVRB and from S-nitroso-BLVRB to the protein substrate. Inhibits insulin signaling by mediating nitrosylation of INSR and IRS1, leading to their inhibition.
Protein Name Flavin Reductase
Nadph
Fr
Biliverdin Reductase B
Bvr-B
Biliverdin-Ix Beta-Reductase
Green Heme-Binding Protein
Ghbp
Nadph-Dependent Diaphorase
Nadph-Flavin Reductase
Flr
S-Nitroso-Coa-Assisted Nitrosyltransferase
Sno-Coa-Assisted Nitrosyltransferase
Database Links Reactome: R-HSA-189483
Reactome: R-HSA-9707564
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Flavin Reductase antibody
Anti-Nadph antibody
Anti-Fr antibody
Anti-Biliverdin Reductase B antibody
Anti-Bvr-B antibody
Anti-Biliverdin-Ix Beta-Reductase antibody
Anti-Green Heme-Binding Protein antibody
Anti-Ghbp antibody
Anti-Nadph-Dependent Diaphorase antibody
Anti-Nadph-Flavin Reductase antibody
Anti-Flr antibody
Anti-S-Nitroso-Coa-Assisted Nitrosyltransferase antibody
Anti-Sno-Coa-Assisted Nitrosyltransferase antibody
Anti-BLVRB antibody
Anti-FLR antibody
Anti-SCAN antibody

Information sourced from Uniprot.org

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