Host: | Mouse |
Applications: | FC/IF/IHC |
Reactivity: | Human |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | Mouse monoclonal antibody anti-Blood Group A-CD173 is suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications. |
Clonality : | Monoclonal |
Clone ID : | 3-3A |
Conjugation: | Unconjugated |
Isotype: | IgG1k |
Formulation: | PBS with 0.02% Sodium Azide. |
Purification: | Affinity purified from tissue culture. |
Concentration: | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
Dilution Range: | Flow Cytometry (0, 5 – 1, 0 µg/million cells in 0, 1 ml). Immunofluorescence (0, 5 – 1, 0 µg/ml). Immunohistology (formalin-fixed: 1-2 µg/ml for30 min at RT; requires boiling tissue sections in 10mM Citrate Buffer, pH 6, 0, for 10-20 min followed by |
Storage Instruction: | Store for up to 1 year at 2-8°C upon receipt. |
Immunogen: | A BALB/c mouse was immunized with mucin isolated from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification). Fusion partner: SP2/0. |
Background | 3-3A reacts with determinants of chain A type 3 and 4 and chain H type 3 and 4, but not with type 1 and 2 chain structures. It is not reactive with immunodominant A trisaccharide. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas. 3-3A does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues in these individuals. |
Information sourced from Uniprot.org