• STJ70040 (0. 3µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70040 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing some membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70040 Flow cytometric analysis of paraformaldehyde fixed Daudi cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-BLNK/SLP-65 antibody (C-Term) (STJ70040)

SKU:
STJ70040-100

£312.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-BLNK/SLP-65 (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:32000.
WB: Approx 70-75kDa band observed in lysates of cell line Daudi (calculated MW of 50.5kDa according to NP_037446.1). This molecular weight is routinely observed by other sources. Recommend
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: BLNK
Gene ID: 29760
Uniprot ID: BLNK_HUMAN
Immunogen Region: C-Term
Accession Number: NP_037446.1; NP_001107566.1; NP_001245369.1; NP_001245370.1; NP_001245371.1
Specificity: This antibody is expected to recognize all reported isoforms.
Immunogen Sequence: KDSTRLKYAVKVS
Post Translational Modifications Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways.
Function Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.
Protein Name B-Cell Linker Protein
B-Cell Adapter Containing A Sh2 Domain Protein
B-Cell Adapter Containing A Src Homology 2 Domain Protein
Cytoplasmic Adapter Protein
Src Homology 2 Domain-Containing Leukocyte Protein Of 65 Kda
Slp-65
Database Links Reactome: R-HSA-912631
Reactome: R-HSA-9679191
Reactome: R-HSA-983695
Cellular Localisation Cytoplasm
Cell Membrane
Bcr Activation Results In The Translocation To Membrane Fraction
Alternative Antibody Names Anti-B-Cell Linker Protein antibody
Anti-B-Cell Adapter Containing A Sh2 Domain Protein antibody
Anti-B-Cell Adapter Containing A Src Homology 2 Domain Protein antibody
Anti-Cytoplasmic Adapter Protein antibody
Anti-Src Homology 2 Domain-Containing Leukocyte Protein Of 65 Kda antibody
Anti-Slp-65 antibody
Anti-BLNK antibody
Anti-BASH antibody
Anti-SLP65 antibody

Information sourced from Uniprot.org

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