| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-biotinidase (372-385) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 60kDa band observed in Human Liver lysates (calculated MW of 61.1kDa according to NP_000051.1). Recommended concentration: 0.5-1.5µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | BTD |
| Gene ID: | 686 |
| Uniprot ID: | BTD_HUMAN |
| Immunogen Region: | 372-385 |
| Accession Number: | NP_000051.1 |
| Immunogen Sequence: | EKDAQEVHCDEATK |
| Protein Name | BiotinidaseBiotinase |
| Database Links | Reactome: R-HSA-196780Reactome: R-HSA-3371598 |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative Antibody Names | Anti-Biotinidase antibodyAnti-Biotinase antibodyAnti-BTD antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and macroH2A. 1 knockout (KO) 293T cells, using [KO Validated] macroH2A. 1 Rabbit polyclonal antibody (STJ11100064) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89134/357583/STJ11100064_1__90999.1713121716.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and macroH2A. 1 knockout (KO) 293T cells, using [KO Validated] macroH2A. 1 Rabbit polyclonal antibody (STJ11100064) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
