Anti-Biotin antibody [Hyb-8] (STJ16100225)

Anti-Biotin antibody [Hyb-8] (STJ16100225)

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STJ16100225
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Host: Mouse
Applications: ELISA/FC/WB/IF/IHC
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Mouse monoclonal antibody anti-Biotin is suitable for use in ELISA, Flow Cytometry, Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality : Monoclonal
Clone ID : Hyb-8
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: PBS with 0.02% Sodium Azide.
Purification: Affinity purified from tissue culture.
Concentration: Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL.
Dilution Range: ELISA (solid phase; not known; tracer: 0, 001-100 µg/ml for 30 min at RT). Flow cytometry (0, 5-1, 0 µg/million cells in 0, 1 ml). Immunoblotting (0, 5-1, 0 µg/ml). Immunofluorescence (0, 5-1, 0 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT).
Storage Instruction: Store for up to 1 year at 2-8°C upon receipt.
Gene Symbol: HLCS
Gene ID: 3141
Uniprot ID: BPL1_HUMAN
Immunogen: A BALB/c mice were immunized with biotinylated sheep immunoglobulin while NHS-biotin was used as a biotinylation agent. Fusion partner: SP2/0.
Tissue Specificity Widely expressed. Mostly expressed in muscle, placenta and to a lower extent in the brain, kidney, pancreas, liver and lung.
Function Biotin--protein ligase catalyzing the biotinylation of the 4 biotin-dependent carboxylases acetyl-CoA-carboxylase, pyruvate carboxylase, propionyl-CoA carboxylase, and methylcrotonyl-CoA carboxylase.
Protein Name Biotin--Protein Ligase
Biotin Apo-Protein Ligase Includes - Biotin--Methylmalonyl-Coa-Carboxytransferase Ligase - Biotin--Propionyl-Coa-Carboxylase Atp-Hydrolyzing Ligase
Holocarboxylase Synthetase
Hcs - Biotin--Methylcrotonoyl-Coa-Carboxylase Ligase - Biotin--Acetyl-Coa-Carboxylase Ligase
Database Links Reactome: R-HSA-196780
Reactome: R-HSA-3371599
Cellular Localisation Cytoplasm
Mitochondrion
Alternative Antibody Names Anti-Biotin--Protein Ligase antibody
Anti-Biotin Apo-Protein Ligase Includes - Biotin--Methylmalonyl-Coa-Carboxytransferase Ligase - Biotin--Propionyl-Coa-Carboxylase Atp-Hydrolyzing Ligase antibody
Anti-Holocarboxylase Synthetase antibody
Anti-Hcs - Biotin--Methylcrotonoyl-Coa-Carboxylase Ligase - Biotin--Acetyl-Coa-Carboxylase Ligase antibody
Anti-HLCS antibody

Information sourced from Uniprot.org

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