| Host: | Rabbit |
| Applications: | WB/IF/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Osteocalcin (31-80 aa) is suitable for use in Western Blot, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BGLAP |
| Gene ID: | 632 |
| Uniprot ID: | OSTCN_HUMAN |
| Immunogen Region: | 31-80 aa |
| Specificity: | OSTCN Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: | Synthesized peptide derived from part of the human protein at the amino acid range 31-80 |
| Function | Bone protein that constitutes 1-2% of the total bone protein, and which acts as a negative regulator of bone formation. Functions to limit bone formation without impairing bone resorption or mineralization. It binds strongly to apatite and calcium. The uncarboxylated form acts as a hormone secreted by osteoblasts, which regulates different cellular processes, such as energy metabolism, male fertility and brain development. Regulates of energy metabolism by acting as a hormone favoring pancreatic beta-cell proliferation, insulin secretion and sensitivity and energy expenditure. Uncarboxylated osteocalcin hormone also promotes testosterone production in the testes: acts as a ligand for G protein-coupled receptor GPRC6A at the surface of Leydig cells, initiating a signaling response that promotes the expression of enzymes required for testosterone synthesis in a CREB-dependent manner. Also acts as a regulator of brain development: osteocalcin hormone crosses the blood-brain barrier and acts as a ligand for GPR158 on neurons, initiating a signaling response that prevents neuronal apoptosis in the hippocampus, favors the synthesis of all monoamine neurotransmitters and inhibits that of gamma-aminobutyric acid (GABA). Osteocalcin also crosses the placenta during pregnancy and maternal osteocalcin is required for fetal brain development. |
| Protein Name | Osteocalcin Bone Gla Protein Bgp Gamma-Carboxyglutamic Acid-Containing Protein |
| Database Links | Reactome: R-HSA-159740 Reactome: R-HSA-159763 Reactome: R-HSA-159782 Reactome: R-HSA-8940973 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Osteocalcin antibody Anti-Bone Gla Protein antibody Anti-Bgp antibody Anti-Gamma-Carboxyglutamic Acid-Containing Protein antibody Anti-BGLAP antibody |
Information sourced from Uniprot.org