HeLa lysate either in RIPA buffer (lanes 1, 2, 4, 6) or TX100 buffer (lanes 3, 5, 7) overexpressing Mouse ßCstf-64 (DYKDDDDK tagged) immunoprecipitated using STJ72911 (2ug) with Protein G-coated magnetic beads in lanes 6 and 7 and probed with anti-DYKDDDDK (1/1000). Mock-transfected HeLa in Lane 1. A 1/40th part of the lysate was loaded in lanes 2 and 3 before IP and in lanes 4 an 5 after IP. Primary incubations were for 1 hour. Detected by chemiluminescence. Data obtained from Dr P Grozdanov, Texas Tech University Health Sciences Center, USA.

Anti-betaCstF-64 variant 1/betaCstF-64 variant 3-Mouse antibody (Internal) (STJ72911)

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STJ72911-100
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Host: Goat
Applications: Pep-ELISA/IHC/IP
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Goat polyclonal antibody anti-betaCstF-64 variant 1/betaCstF-64 variant 3-Mouse (Internal) is suitable for use in ELISA, Immunohistochemistry and Immunoprecipitation research applications.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:128000.
WB: IP: In transfected HeLa transiently expressing Mouse ßCstf-64 ( (DYKDDDDK tagged) , a band of approx. 80kDa was observed using anti-DYKDDDDK antibody in Western blot after immunopreci
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Immunogen Region: Internal
Accession Number: ACF05702.1; ACF05700.1
Specificity: This antibody recognizes mouse variants 1 and 3 of the reported beta isoform (ACF05702.1; ACF05700.1).
Immunogen Sequence: PASIERVQGQRT
Function One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs.
Protein Name Cleavage Stimulation Factor Subunit 2
Cf-1 64 Kda Subunit
Cleavage Stimulation Factor 64 Kda Subunit
Cstf 64 Kda Subunit
Cstf-64
Database Links Reactome: R-MMU-72163
Reactome: -MMU-72187
Reactome: -MMU-73856
Reactome: -MMU-77595
Cellular Localisation Nucleus
Localized With Ddx1 In Cleavage Bodies
Alternative Antibody Names Anti-Cleavage Stimulation Factor Subunit 2 antibody
Anti-Cf-1 64 Kda Subunit antibody
Anti-Cleavage Stimulation Factor 64 Kda Subunit antibody
Anti-Cstf 64 Kda Subunit antibody
Anti-Cstf-64 antibody
Anti-Cstf2 antibody

Information sourced from Uniprot.org

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