Anti-Beta 1 Integrin antibody [BV7] (STJ16100152)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
General Information
| Short Description | Mouse monoclonal anti-Beta 1 Integrin for use in FC, ELISA, IP and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/ELISA/IP/WB |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | BV7 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | 100 Mu g/mL |
| Dilution Range | FC 1:50ELISA 1:50WB 1:50IP 1:50 |
| Formulation | PBS with 0.1% BSA and 0.02% sodium azide |
| Storage Instruction | Store at 2-8Β°C for up to 1-year, upon receipt. |
Target Information
Additional Info
| Background | The monoclonal antibody BV7 recognizes human ΓΕΈ1-integrin. Beta-1 integrin is a ubiquitously expressed ~89 kDa type I transmembrane protein functioning as receptor when heterodimerized with one alpha subunit. It belongs to the integrin beta chain family consisting of four different genes, encoding multiple ΓΒ²-integrins via alternative splicing. Ligand-recognition depends on the composition of the heterodimer: either collagen, fibronectin, VCAM1, laminin, cytotactin, osteopontin, epiligrin, thrombospondin and CSPG4 can bind to the integrin-complex. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi s sarcoma lesions. When associated with Alpha 7, ΓΒ²1-integrin regulates cell adhesion and laminin matrix deposition. BV7 is active on HT-29 colon carcinoma cells and on HCCP-2998 tumor cells. It is involved in promoting endothelial cell motility and angiogenesis. Furthermore, ΓΒ²1-integrin plays a mechanistic adhesive role during telophase, and is required for the successful completion of cytokinesis. Upon activation integrins in general, including ΓΒ²1-integrin, are known to exhibit global structural rearrangements and exposure of ligand binding sites. ΓΒ²1-integrin modulation is of importance in tissue repair and regeneration. In cultured primary hippocampal neurons, astrocytes and tissues, cell surface expression of amyloid beta fibrils (key hallmark of AlzheimerΓ’β¬β’s disease) selectively co-localized with ΓΒ²1-integrin. Preincubation of cells with antibodies against ΓΒ²1-integrin, as well as Alpha 1-integrin, greatly enhanced amyloid beta-induced apoptosis, indicating a protective role for integrins in apoptosis. The monoclonal antibody BV7 does not recognize Alpha 5ΓΒ²1 complex and not the cytoplasmic part of the ΓΒ²1-subunit. Monoclonal antibody BV7 is active on HT-29 colon carcinoma cells and on HCCP-2998 tumor cells. BV7 binds to several other tumor cells (MG3 osteosarcoma, A375 melanoma, MHCC-1410 and Lovo colon carcinoma) but does not affect adhesion to endothelial cells. |
Information sourced from Uniprot.org