| Host: | Rabbit |
| Applications: | CM/ELISA/IF/IHC/IP/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-BEST1 (500-585) is suitable for use in Confocal Microscopy, ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Biotin |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.75 µg/µl |
| Dilution Range: | WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 IP: 1:200 IHC: 1:100 ICC: 1:100 IF: 1:100 CFM: 1:100 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | BEST1 |
| Gene ID: | 7439 |
| Uniprot ID: | BEST1_HUMAN |
| Immunogen Region: | 500-585 |
| Immunogen: | Synthetic peptide taken within amino acid region 500-585 on human Bestrophin-1 isoform 1 protein. This peptide will label the Bestrophin 1-1-2,-3,-4,-5 isoforms that are produced by alternate splicing of the C-terminal end. |
| Tissue Specificity | Predominantly expressed in the basolateral membrane of the retinal pigment epithelium. |
| Function | Ligand-gated anion channel that allows the movement of anions across cell membranes when activated by calcium (Ca2+). Allows the movement of chloride and hydrogencarbonate. Found in a partially open conformation leading to significantly smaller chloride movement. Upon F2R/PAR-1 activation, the sequestered calcium is released into the cytosol of astrocytes, leading to the (Ca2+)-dependent release of L-glutamate into the synaptic cleft that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation. Upon activation of the norepinephrine-alpha-1 adrenergic receptor signaling pathway, transports as well D-serine than L-glutamate in a (Ca2+)-dependent manner, leading to activation of adjacent NMDAR receptors and therefore regulates the heterosynaptic long-term depression and metaplasticity during initial memory acquisition. Releases the 4-aminobutanoate neurotransmitter in a (Ca2+)-dependent manner, and participates in its tonic release from cerebellar glial cells. |
| Protein Name | Bestrophin-1 Tu15b Vitelliform Macular Dystrophy Protein 2 |
| Database Links | Reactome: R-HSA-2672351 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Basolateral Cell Membrane Localized At The Surface Membrane Of Microdomains Adjacent To Glutamatergic Synapses |
| Alternative Antibody Names | Anti-Bestrophin-1 antibody Anti-Tu15b antibody Anti-Vitelliform Macular Dystrophy Protein 2 antibody Anti-BEST1 antibody Anti-VMD2 antibody |
Information sourced from Uniprot.org