| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Beclin-1 (1-280) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:100IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
BECN1 |
| Gene ID: |
8678 |
| Uniprot ID: |
BECN1_HUMAN |
| Immunogen Region: |
1-280 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Beclin 1 (NP_003757.1). |
| Immunogen Sequence: |
MEGSKTSNNSTMQVSFVCQR CSQPLKLDTSFKILDRVTIQ ELTAPLLTTAQAKPGETQEE ETNSGEEPFIETPRQDGVSR RFIPPARMMSTESANSFTLI GEASDGGTMENLSRRLKVTG DLFDIMSGQTDVDHPLCEEC TDTLLDQLDTQLNVTENECQ NYKRCLEILEQMNEDDSEQL QMELKELALEEERLIQELED VEKNRKIVAENLEKVQAEAE RLDQEEAQYQREYSEFKRQ |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy. In response to autophagic stimuli, phosphorylated at serine residues by AMPK in an ATG14-dependent manner, and this phosphorylation is critical for maximally efficient autophagy. Polyubiquitinated by NEDD4, both with 'Lys-11'- and 'Lys-63'-linkages. 'Lys-11'-linked polyubiquitination leads to degradation and is enhanced when the stabilizing interaction partner VPS34 is depleted. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes. Polyubiquitinated at Lys-402 with 'Lys-48'-linkages. 'Lys-48'-linked polyubiquitination of Lys-402 leads to degradation. Deubiquitinated by ATXN3, leading to stabilization. Ubiquitinated at Lys-437 via 'Lys-63'-linkage by the DCX(AMBRA1) complex, thereby increasing the association between BECN1 and PIK3C3 to promote PIK3C3 activity. 'Lys-48'-linked ubiquitination by RNF216 leads to proteasomal degradation and autophagy inhibition. Proteolytically processed by caspases including CASP8 and CASP3.the C-terminal fragments lack autophagy-inducing capacity and are proposed to induce apoptosis. Thus the cleavage is proposed to be an determinant to switch from autophagy to apoptosis pathways affecting cellular homeostasis including viral infections and survival of tumor cells. |
| Function | Plays a central role in autophagy. Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate.different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis. Protects against infection by a neurovirulent strain of Sindbis virus. May play a role in antiviral host defense. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis.it induces the mitochondrial translocation of BAX and the release of proapoptotic factors. |
| Protein Name | Beclin-1Coiled-Coil Myosin-Like Bcl2-Interacting ProteinProtein Gt197 Cleaved Into - Beclin-1-C 35 Kda - Beclin-1-C 37 Kda |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-5689880Reactome: R-HSA-9679504Reactome: R-HSA-9694676Reactome: R-HSA-9705671Reactome: R-HSA-983170 |
| Cellular Localisation | CytoplasmGolgi ApparatusTrans-Golgi Network MembranePeripheral Membrane ProteinEndosome MembraneEndoplasmic Reticulum MembraneMitochondrion MembraneEndosomeCytoplasmic VesicleAutophagosomeInteraction With Atg14 Promotes Translocation To AutophagosomesExpressed In Dendrites And Cell Bodies Of Cerebellar Purkinje CellsBeclin-1-C 35 Kda: MitochondrionNucleusBeclin-1-C 37 Kda: Mitochondrion |
| Alternative Antibody Names | Anti-Beclin-1 antibodyAnti-Coiled-Coil Myosin-Like Bcl2-Interacting Protein antibodyAnti-Protein Gt197 Cleaved Into - Beclin-1-C 35 Kda - Beclin-1-C 37 Kda antibodyAnti-BECN1 antibodyAnti-GT197 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
