• Immunofluorescence analysis of PC-12 cells using [KO Validated] Beclin 1 Rabbit polyclonal antibody (STJ11104484) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Beclin 1 Rabbit polyclonal antibody (STJ11104484) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Beclin 1 in paraffin-embedded mouse kidney using [KO Validated] Beclin 1 Rabbit polyclonal antibody (STJ11104484) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Beclin 1 in paraffin-embedded human stomach using [KO Validated] Beclin 1 Rabbit polyclonal antibody (STJ11104484) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse lung using [KO Validated] Beclin 1 Rabbit polyclonal antibody (STJ11104484) at 1:900 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:60s.
  • Western blot analysis of lysates from wild type (WT) and Beclin 1 knockout (KO) 239T cells using [KO Validated] Beclin 1 Rabbit polyclonal antibody (STJ11104484) at 1:900 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 60s.

Anti-BECN1 antibody (1-280) (STJ11104484)

SKU:
STJ11104484

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Beclin-1 (1-280) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BECN1
Gene ID: 8678
Uniprot ID: BECN1_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Beclin 1 (NP_003757.1).
Immunogen Sequence: MEGSKTSNNSTMQVSFVCQR CSQPLKLDTSFKILDRVTIQ ELTAPLLTTAQAKPGETQEE ETNSGEEPFIETPRQDGVSR RFIPPARMMSTESANSFTLI GEASDGGTMENLSRRLKVTG DLFDIMSGQTDVDHPLCEEC TDTLLDQLDTQLNVTENECQ NYKRCLEILEQMNEDDSEQL QMELKELALEEERLIQELED VEKNRKIVAENLEKVQAEAE RLDQEEAQYQREYSEFKRQ
Tissue Specificity Ubiquitous.
Post Translational Modifications Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy. In response to autophagic stimuli, phosphorylated at serine residues by AMPK in an ATG14-dependent manner, and this phosphorylation is critical for maximally efficient autophagy. Polyubiquitinated by NEDD4, both with 'Lys-11'- and 'Lys-63'-linkages. 'Lys-11'-linked polyubiquitination leads to degradation and is enhanced when the stabilizing interaction partner VPS34 is depleted. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes. Polyubiquitinated at Lys-402 with 'Lys-48'-linkages. 'Lys-48'-linked polyubiquitination of Lys-402 leads to degradation. Deubiquitinated by ATXN3, leading to stabilization. Ubiquitinated at Lys-437 via 'Lys-63'-linkage by the DCX(AMBRA1) complex, thereby increasing the association between BECN1 and PIK3C3 to promote PIK3C3 activity. 'Lys-48'-linked ubiquitination by RNF216 leads to proteasomal degradation and autophagy inhibition. Proteolytically processed by caspases including CASP8 and CASP3.the C-terminal fragments lack autophagy-inducing capacity and are proposed to induce apoptosis. Thus the cleavage is proposed to be an determinant to switch from autophagy to apoptosis pathways affecting cellular homeostasis including viral infections and survival of tumor cells.
Function Plays a central role in autophagy. Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate.different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis. Protects against infection by a neurovirulent strain of Sindbis virus. May play a role in antiviral host defense. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis.it induces the mitochondrial translocation of BAX and the release of proapoptotic factors.
Protein Name Beclin-1
Coiled-Coil Myosin-Like Bcl2-Interacting Protein
Protein Gt197 Cleaved Into - Beclin-1-C 35 Kda - Beclin-1-C 37 Kda
Database Links Reactome: R-HSA-1632852
Reactome: R-HSA-5689880
Reactome: R-HSA-9679504
Reactome: R-HSA-9694676
Reactome: R-HSA-9705671
Reactome: R-HSA-983170
Cellular Localisation Cytoplasm
Golgi Apparatus
Trans-Golgi Network Membrane
Peripheral Membrane Protein
Endosome Membrane
Endoplasmic Reticulum Membrane
Mitochondrion Membrane
Endosome
Cytoplasmic Vesicle
Autophagosome
Interaction With Atg14 Promotes Translocation To Autophagosomes
Expressed In Dendrites And Cell Bodies Of Cerebellar Purkinje Cells
Beclin-1-C 35 Kda: Mitochondrion
Nucleus
Beclin-1-C 37 Kda: Mitochondrion
Alternative Antibody Names Anti-Beclin-1 antibody
Anti-Coiled-Coil Myosin-Like Bcl2-Interacting Protein antibody
Anti-Protein Gt197 Cleaved Into - Beclin-1-C 35 Kda - Beclin-1-C 37 Kda antibody
Anti-BECN1 antibody
Anti-GT197 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance