| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S4814RM |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:10000-1:30000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunogen Region: | 1-280 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Beclin 1 (NP_003757.1). |
| Immunogen Sequence: | MEGSKTSNNSTMQVSFVCQR CSQPLKLDTSFKILDRVTIQ ELTAPLLTTAQAKPGETQEE ETNSGEEPFIETPRQDGVSR RFIPPARMMSTESANSFTLI GEASDGGTMENLSRRLKVTG DLFDIMSGQTDVDHPLCEEC TDTLLDQLDTQLNVTENECQ NYKRCLEILEQMNEDDSEQL QMELKELALEEERLIQELED VEKNRKIVAENLEKVQAEAE RLDQEEAQYQREYSEFKRQ |
| Background | Beclin 1 plays a central role in autophagy. It acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis.There two casp cleavage sites in Beclin 1, TDVD (133) and DQLD (149) , cleavage at which yields fragments lacking the autophagy-inducing capacity. Noteworthy, the C-terminal fragment, Beclin 1-C, localized predominantly at the mitochondria and sensitized the cells to apoptosis. It induces the mitochondrial translocation of BAX and the release of proapoptotic factors. |
Information sourced from Uniprot.org