| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BDNF |
| Gene ID: | 627 |
| Uniprot ID: | BDNF_HUMAN |
| Immunogen Region: | 20-247 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-247 of human BDNF (NP_733928.1). |
| Immunogen Sequence: | PMKEANIRGQGGLAYPGVRT HGTLESVNGPKAGSRGLTSL ADTFEHVIEELLDEDQKVRP NEENNKDADLYTSRVMLSSQ VPLEPPLLFLLEEYKNYLDA ANMSMRVRRHSDPARRGELS VCDSISEWVTAADKKTAVDM SGGTVTVLEKVPVSKGQLKQ YFYETKCNPMGYTKEGCRGI DKRHWNSQCRTTQSYVRALT MDSKKRIGWRFIRIDTSCVC TLTIKRGR |
| Tissue Specificity | Detected in blood plasma and in saliva (at protein level). Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta. |
| Post Translational Modifications | Neurotrophic factor BDNF precursor form: N-glycosylated and glycosulfated, contrary to mature BDNF. Mature BDNF is produced by proteolytic removal of the propeptide, catalyzed by a FURIN family member. In addition, the precursor form is proteolytically cleaved within the propeptide, but this is not an obligatory intermediate for the production of mature BDNF. Can be converted into mature BDNF by plasmin (PLG). |
| Function | Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. Neurotrophic factor BDNF precursor form: Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse. |
| Protein Name | Neurotrophic Factor Bdnf Precursor Form Probdnf Abrineurin Brain-Derived Neurotrophic Factor Cleaved Into - Neurotrophic Factor Bdnf |
| Database Links | Reactome: R-HSA-1257604 Reactome: R-HSA-2219530 Reactome: R-HSA-6811558 Reactome: R-HSA-9022702 Reactome: R-HSA-9024909 Reactome: R-HSA-9026519 Reactome: R-HSA-9026527 Reactome: R-HSA-9028335 Reactome: R-HSA-9028731 Reactome: R-HSA-9032500 Reactome: R-HSA-9032759 Reactome: R-HSA-9032845 Reactome: R-HSA-9768919 |
| Cellular Localisation | Secreted Neurotrophic Factor Bdnf Precursor Form: Secreted A Proportion Of Bdnf Is Secreted As Immature Precursor (Probdnf) |
| Alternative Antibody Names | Anti-Neurotrophic Factor Bdnf Precursor Form antibody Anti-Probdnf antibody Anti-Abrineurin antibody Anti-Brain-Derived Neurotrophic Factor Cleaved Into - Neurotrophic Factor Bdnf antibody Anti-BDNF antibody |
Information sourced from Uniprot.org