| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-BDNF (20-247) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
BDNF |
| Gene ID: |
627 |
| Uniprot ID: |
BDNF_HUMAN |
| Immunogen Region: |
20-247 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 20-247 of human BDNF (NP_733928.1). |
| Immunogen Sequence: |
PMKEANIRGQGGLAYPGVRT HGTLESVNGPKAGSRGLTSL ADTFEHVIEELLDEDQKVRP NEENNKDADLYTSRVMLSSQ VPLEPPLLFLLEEYKNYLDA ANMSMRVRRHSDPARRGELS VCDSISEWVTAADKKTAVDM SGGTVTVLEKVPVSKGQLKQ YFYETKCNPMGYTKEGCRGI DKRHWNSQCRTTQSYVRALT MDSKKRIGWRFIRIDTSCVC TLTIKRGR |
| Tissue Specificity | Detected in blood plasma and in saliva (at protein level). Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta. |
| Post Translational Modifications | BDNF precursor form: N-glycosylated and glycosulfated, contrary to mature BDNF. Mature BDNF is produced by proteolytic removal of the propeptide, catalyzed by a FURIN family member. In addition, the precursor form is proteolytically cleaved within the propeptide, but this is not an obligatory intermediate for the production of mature BDNF. Can be converted into mature BDNF by plasmin (PLG). |
| Function | Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. BDNF precursor form: Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse. |
| Protein Name | Brain-Derived Neurotrophic FactorBdnfAbrineurin Cleaved Into - Bdnf Precursor FormProbdnf |
| Database Links | Reactome: R-HSA-9022702Reactome: R-HSA-9024909Reactome: R-HSA-9026519Reactome: R-HSA-9026527Reactome: R-HSA-9028335Reactome: R-HSA-9028731Reactome: R-HSA-9032500Reactome: R-HSA-9032759Reactome: R-HSA-9032845Reactome: R-HSA-9768919 |
| Cellular Localisation | SecretedBdnf Precursor Form: SecretedA Proportion Of Bdnf Is Secreted As Immature Precursor (Probdnf) |
| Alternative Antibody Names | Anti-Brain-Derived Neurotrophic Factor antibodyAnti-Bdnf antibodyAnti-Abrineurin Cleaved Into - Bdnf Precursor Form antibodyAnti-Probdnf antibodyAnti-BDNF antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
