| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BCL9 |
| Gene ID: | 607 |
| Uniprot ID: | BCL9_HUMAN |
| Immunogen Region: | 1-100 aa |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human Bcl9. (NP_004317.2). |
| Immunogen Sequence: | MHSSNPKVRSSPSGNTQSSP KSKQEVMVRPPTVMSPSGNP QLDSKFSNQGKQGGSASQSQ PSPCDSKSGGHTPKALPGPG GSMGLKNGAGNGAKGKGKRE |
| Tissue Specificity | Detected at low levels in thymus, prostate, testis, ovary and small intestine, and at lower levels in spleen, colon and blood. |
| Function | Involved in signal transduction through the Wnt pathway. Promotes beta-catenin's transcriptional activity. |
| Protein Name | B-Cell Cll/Lymphoma 9 ProteinB-Cell Lymphoma 9 ProteinBcl-9Protein Legless Homolog |
| Database Links | Reactome: R-HSA-201722Reactome: R-HSA-3769402 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-B-Cell Cll/Lymphoma 9 Protein antibodyAnti-B-Cell Lymphoma 9 Protein antibodyAnti-Bcl-9 antibodyAnti-Protein Legless Homolog antibodyAnti-BCL9 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and Bcl9 knockdown (KD) 293T cells using [KD Validated] Bcl9 Rabbit polyclonal antibody (STJ11105603) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/178247/370554/STJ11105603_1__32101.1713136505.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Bcl9 knockdown (KD) 293T cells using [KD Validated] Bcl9 Rabbit polyclonal antibody (STJ11105603) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
