• Western blot analysis of extracts of various cell lines, using BCL2L12 rabbit monoclonal antibody (STJ11103149) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using BCL2L12 rabbit monoclonal antibody (STJ11103149) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunoprecipitation analysis of 300 Mu g extracts of PC-3 cells using 3 Mu g BCL2L12 antibody (STJ11103149). Western blot was performed from the immunoprecipitate using BCL2L12 antibody (STJ11103149) at a dilution of 1:500.

Anti-BCL2L12 antibody (200-300) [S9MR] (STJ11103149)

SKU:
STJ11103149

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IHC-P/IP/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCL2L12
Gene ID: 83596
Uniprot ID: B2L12_HUMAN
Immunogen Region: 200-300
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human BCL2L12 (Q9HB09).
Immunogen Sequence: GPPSTEKEAILRRLVALLEE EAEVINQKLASDPALRSKLV RLSSDSFARLVELFCSRDDS SRPSRACPGPPPPSPEPLAR LALAMELSRRVAGLGGTLAG L
Tissue Specificity Expressed mainly in breast, thymus, prostate, fetal liver, colon, placenta, pancreas, small intestine, spinal cord, kidney, and bone marrow and to a lesser extent in many other tissues. Isoform 2 is primarily expressed in skeletal muscle.
Protein Name Bcl-2-Like Protein 12
Bcl2-L-12
Bcl-2-Related Proline-Rich Protein
Alternative Antibody Names Anti-Bcl-2-Like Protein 12 antibody
Anti-Bcl2-L-12 antibody
Anti-Bcl-2-Related Proline-Rich Protein antibody
Anti-BCL2L12 antibody
Anti-BPR antibody

Information sourced from Uniprot.org

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