| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BCL2L11 |
| Gene ID: | 10018 |
| Uniprot ID: | B2L11_HUMAN |
| Immunogen Region: | 100-198 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 100-198 of human Bim (NP_619527.1). |
| Immunogen Sequence: | DTDRSPAPMSCDKSTQTPSP PCQAFNHYLSAMASMRQAEP ADMRPEIWIAQELRRIGDEF NAYYARRVFLNNYQAAEDHP RMVILRLLRYIVRLVWRMH |
| Tissue Specificity | Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are widely expressed with tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. |
| Post Translational Modifications | Phosphorylation at Ser-69 by MAPK1/MAPK3 leads to interaction with TRIM2 and polyubiquitination, followed by proteasomal degradation. Deubiquitination catalyzed by USP27X stabilizes the protein. Ubiquitination by TRIM2 following phosphorylation by MAPK1/MAPK3 leads to proteasomal degradation. Conversely, deubiquitination catalyzed by USP27X stabilizes the protein. |
| Function | Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis. |
| Protein Name | Bcl-2-Like Protein 11 Bcl2-L-11 Bcl2-Interacting Mediator Of Cell Death |
| Database Links | Reactome: R-HSA-111446 Reactome: R-HSA-111453 Reactome: R-HSA-193648 Reactome: R-HSA-6802952 Reactome: R-HSA-8862803 Reactome: R-HSA-8952158 Reactome: R-HSA-9607240 Reactome: R-HSA-9614657 |
| Cellular Localisation | Endomembrane System Peripheral Membrane Protein Associated With Intracytoplasmic Membranes Isoform Bimel: Mitochondrion Translocates From Microtubules To Mitochondria On Loss Of Cell Adherence Isoform Biml: Mitochondrion Isoform Bims: Mitochondrion Isoform Bim-Alpha1: Mitochondrion |
| Alternative Antibody Names | Anti-Bcl-2-Like Protein 11 antibody Anti-Bcl2-L-11 antibody Anti-Bcl2-Interacting Mediator Of Cell Death antibody Anti-BCL2L11 antibody Anti-BIM antibody |
Information sourced from Uniprot.org