| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-BAP/SIL1 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 55-60 kDa band observed in Human Liver and Kidney extracts (predicted MW of 53kDa according to NP_071909.1). Recommended concentration: 1-2µg/ml.IHC: Paraffin embedded Human |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | SIL1 |
| Gene ID: | 64374 |
| Uniprot ID: | SIL1_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_071909.1; NP_001032722.1 |
| Specificity: | Variants (NP_071909.1; NP_001032722.1) encode the same isoform. |
| Immunogen Sequence: | ELLGSVNSLLKELR |
| Post Translational Modifications | N-glycosylated. Ubiquitinated by the CRL2(FEM1A) and CRL2(FEM1C) complexes, which recognize the -Lys-Xaa-Xaa-Arg C-degron at the C-terminus, leading to its degradation. |
| Function | Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5. |
| Protein Name | Nucleotide Exchange Factor Sil1Bip-Associated ProteinBap |
| Cellular Localisation | Endoplasmic Reticulum Lumen |
| Alternative Antibody Names | Anti-Nucleotide Exchange Factor Sil1 antibodyAnti-Bip-Associated Protein antibodyAnti-Bap antibodyAnti-SIL1 antibodyAnti-UNQ545 antibodyAnti-PRO836 antibody |
Information sourced from Uniprot.org