• Western blot analysis of extracts of THP-1 cells, using Bak antibody (STJ22759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of C6 cells using Bak rabbit polyclonal antibody (STJ22759) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using Bak rabbit polyclonal antibody (STJ22759) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300 Mu g extracts of THP-1 cells using 3 Mu g Bak antibody (STJ22759). Western blot was performed from the immunoprecipitate using Bak antibody (STJ22759) at a dilution of 1:1000.

Anti-Bak1 antibody (50-150) (STJ22759)

SKU:
STJ22759

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Bak (50-150) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BAK1
Gene ID: 578
Uniprot ID: BAK_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Bak (NP_001179.1).
Immunogen Sequence: EGVAAPADPEMVTLPLQPSS TMGQVGRQLAIIGDDINRRY DSEFQTMLQHLQPTAENAYE YFTKIATSLFESGINWGRVV ALLGFGYRLALHVYQHGLTG F
Tissue Specificity Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.
Function Plays a role in the mitochondrial apoptotic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases.
Protein Name Bcl-2 Homologous Antagonist/Killer
Apoptosis Regulator Bak
Bcl-2-Like Protein 7
Bcl2-L-7
Database Links Reactome: R-HSA-111452
Reactome: R-HSA-111457
Reactome: R-HSA-5620971
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Bcl-2 Homologous Antagonist/Killer antibody
Anti-Apoptosis Regulator Bak antibody
Anti-Bcl-2-Like Protein 7 antibody
Anti-Bcl2-L-7 antibody
Anti-BAK1 antibody
Anti-BAK antibody
Anti-BCL2L7 antibody
Anti-CDN1 antibody

Information sourced from Uniprot.org

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