• Immunohistochemistry analysis of paraffin-embedded rat brain using BAG5 antibody (STJ28358) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using BAG5 antibody (STJ28358) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using BAG5 antibody (STJ28358) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-BAG5 antibody (198-447) (STJ28358)

SKU:
STJ28358

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BAG5 (198-447) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BAG5
Gene ID: 9529
Uniprot ID: BAG5_HUMAN
Immunogen Region: 198-447
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 198-447 of human BAG5 (NP_004864.1).
Immunogen Sequence: GVLIALLMGVNNNETCRHLS CVLSGLIADLDALDVCGRTE IRNYRREVVEDINKLLKYLD LEEEADTTKAFDLRQNHSIL KIEKVLKRMREIKNELLQAQ NPSELYLSSKTELQGLIGQL DEVSLEKNPCIREARRRAVI EVQTLITYIDLKEALEKRKL FACEEHPSHKAVWNVLGNLS EIQGEVLSFDGNRTDKNYIR LEELLTKQLLALDAVDPQGE EKCKAARKQAVRLAQNILS
Tissue Specificity Expressed in the heart.
Function Co-chaperone for HSP/HSP70 proteins. It functions as a nucleotide-exchange factor promoting the release of ADP from HSP70, thereby activating HSP70-mediated protein refolding. Has an essential role in maintaining proteostasis at junctional membrane complexes (JMC), where it may function as a scaffold between the HSPA8 chaperone and JMC proteins enabling correct, HSPA8-dependent JMC protein folding. Inhibits both auto-ubiquitination of PRKN and ubiquitination of target proteins by PRKN.
Protein Name Bag Family Molecular Chaperone Regulator 5
Bag-5
Bcl-2-Associated Athanogene 5
Database Links Reactome: R-HSA-3371453
Cellular Localisation In Cardiomyocytes
Localized At Specialized Membrane Contact Sites Between T-Tubules And The Sarcoplasmic Reticulum
Known As Junctional Membrane Complexes
Alternative Antibody Names Anti-Bag Family Molecular Chaperone Regulator 5 antibody
Anti-Bag-5 antibody
Anti-Bcl-2-Associated Athanogene 5 antibody
Anti-BAG5 antibody
Anti-KIAA0873 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance