• Immunohistochemistry analysis of BAG3 in paraffin-embedded mouse heart using BAG3 Rabbit polyclonal antibody (STJ117026) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of BAG3 in paraffin-embedded human lung using BAG3 Rabbit polyclonal antibody (STJ117026) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of BAG3 in paraffin-embedded rat heart using BAG3 Rabbit polyclonal antibody (STJ117026) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using BAG3 Rabbit polyclonal antibody (STJ117026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BAG3 antibody (380-575) (STJ117026)

SKU:
STJ117026

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BAG3 (380-575) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BAG3
Gene ID: 9531
Uniprot ID: BAG3_HUMAN
Immunogen Region: 380-575
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 380-575 of human BAG3 (NP_004272.2).
Immunogen Sequence: PSAVPSSPKSVATEERAAPS TAPAEATPPKPGEAEAPPKH PGVLKVEAILEKVQGLEQAV DNFEGKKTDKKYLMIEEYLT KELLALDSVDPEGRADVRQA RRDGVRKVQTILEKLEQKAI DVPGQVQVYELQPSNLEADQ PLQAIMEMGAVAADKGKKNA GNAEDPHTETQQPEATAAAT SNPSSMTDTPGNPAAP
Function Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70. Has anti-apoptotic activity. Plays a role in the HSF1 nucleocytoplasmic transport.
Protein Name Bag Family Molecular Chaperone Regulator 3
Bag-3
Bcl-2-Associated Athanogene 3
Bcl-2-Binding Protein Bis
Docking Protein Cair-1
Database Links Reactome: R-HSA-3371453
Cellular Localisation Nucleus
Cytoplasm
Colocalizes With Hsf1 To The Nucleus Upon Heat Stress
Alternative Antibody Names Anti-Bag Family Molecular Chaperone Regulator 3 antibody
Anti-Bag-3 antibody
Anti-Bcl-2-Associated Athanogene 3 antibody
Anti-Bcl-2-Binding Protein Bis antibody
Anti-Docking Protein Cair-1 antibody
Anti-BAG3 antibody
Anti-BIS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance